Anticancer Activity of Eleutherine bulbosa (Mill.) Urb. Extract on WiDr Cell Line In Vitro
- DOI
- 10.2991/phico-17.2018.25How to use a DOI?
- Keywords
- Eleutherine bulbosa (Mill.) Urb.; WiDr cell line; MTT assay; selectivity index
- Abstract
Colon cancer is third of cancer cases that occur in the world's population. The incidence of new cases in men are 614.300 and women are 746.300, while the death rate in men are 373.600 and in women are 320.300. Colon cancer treatments are surgery, chemotherapy, and radiotherapy. The problem with chemotherapy is trigger development of a new drug and among other from natural compound. The plant that is known to have cytotoxic activity against cancer cells are Eleutherine bulbosa (Mill.) Urb. This study aims to analysis the anticancer activity of Eleutherine bulbosa (Mill.) Urb. extract on WiDr cells. The research method includes gradual maceration using n-hexane, ethyl acetate, and ethanol, phytochemical analysis of extracts, cytotoxicity assay on WiDr cells with MTT (Microculture Tetrazolium Technique) assay, and selectivity index assay on WiDr and Vero cells. The MTT assay result show that Eleutherine bulbosa (Mill.) Urb. has cytotoxic activity on WIDr cells, but only ethyl acetate extract are selective. Further studies are to assess the mechanisms of ethyl acetate extracts in induce apoptosis, inhibit the cell cycle, and the expression of protein that associated with it.
- Copyright
- © 2018, the Authors. Published by Atlantis Press.
- Open Access
- This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
Cite this article
TY - CONF AU - Ichwan Alamsyah Lubis AU - M. Farid Ichwan AU - Mustofa Mustofa AU - Denny Satria PY - 2017/12 DA - 2017/12 TI - Anticancer Activity of Eleutherine bulbosa (Mill.) Urb. Extract on WiDr Cell Line In Vitro BT - Proceedings of the 2nd Public Health International Conference (PHICo 2017) PB - Atlantis Press SP - 123 EP - 127 SN - 2468-5739 UR - https://doi.org/10.2991/phico-17.2018.25 DO - 10.2991/phico-17.2018.25 ID - AlamsyahLubis2017/12 ER -