Proceedings of the Conference on Natural Resources And Life Sciences 2022 (NRLS-BIO 2022)

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Bst polymerase enhancement a bioinformatics approach to improve Bst polymerase characteristics

Authors
Jonathan1, Ernest Suryadjaja1, Sulistyo Emantoko D. Putra1, *
1Faculty of Biotechnology, University of Surabaya (UBAYA), Surabaya, 60293, Indonesia
*Corresponding author. Email: emantoko@staff.ubaya.ac.id
Corresponding Author
Sulistyo Emantoko D. Putra
Available Online 30 December 2023.
DOI
10.2991/978-94-6463-322-1_11How to use a DOI?
Keywords
Polymerase; Bst; POCT; Protein Modification
Abstract

DNA polymerase is a remarkably incredible invention in the biotechnology field. Since its discovery, molecular genetic-based research has been growing rapidly. Various methods for molecular-based diagnostics have been developed since. One of which is Loop-Mediated Isothermal Amplification; this method offers simplicity, sensitivity, and a faster result than conventional PCR. However, the availability of the materials supporting the LAMP reaction is still challenging. LAMP materials are often hard to access in a developing country, and the need to import from other countries significantly increases the price. Here we offer a Bst polymerase design that can be produced with the same or even higher quality than the commercial one. Based on bioinformatics analysis, sto7d fusion to the C-terminus of Bst polymerase (Br512g3.1) shows higher stability and solubility. Sto7d fusion to the C-terminus improves processivity when used in Gss (Bst-like polymerase). Lastly, Sto7d fusion to Br512g3.1 offers higher stability and processivity that can be used to overcome the cost problem of Bst polymerase.

Copyright
© 2023 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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Volume Title
Proceedings of the Conference on Natural Resources And Life Sciences 2022 (NRLS-BIO 2022)
Series
Advances in Biological Sciences Research
Publication Date
30 December 2023
ISBN
10.2991/978-94-6463-322-1_11
ISSN
2468-5747
DOI
10.2991/978-94-6463-322-1_11How to use a DOI?
Copyright
© 2023 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

Cite this article

risenwbib
TY  - CONF
AU  - Jonathan
AU  - Ernest Suryadjaja
AU  - Sulistyo Emantoko D. Putra
PY  - 2023
DA  - 2023/12/30
TI  - Bst polymerase enhancement a bioinformatics approach to improve Bst polymerase characteristics
BT  - Proceedings of the Conference on Natural Resources And Life Sciences 2022 (NRLS-BIO 2022)
PB  - Atlantis Press
SP  - 86
EP  - 89
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-322-1_11
DO  - 10.2991/978-94-6463-322-1_11
ID  - 2023
ER  -