A Cotton Bud Coated with Chitosan/N-Carbon Dot Composite as SPE Sorbent for Extraction of Steroid Hormones from Fish Farm Water

DOI

10.2991/978-94-6463-130-2_2How to use a DOI?

Keywords

steroid hormones; N-carbon dot; chitosan; solid phase extraction

Abstract

17-β Estradiol (E2), estrone (E1), and testosterone (T) are steroid hormones formed naturally by humans and animals or derived from synthetic sources. These steroid hormones are frequently used in aquaculture farms to increase production and treat diseases. However, there are concerns about the presence of steroids in farm wastewater since steroid hormones are known to be potent endocrine disruptors even at their lowest concentration. Therefore, it necessitates the development of an analytical method for monitoring these hormones in fish farms. This work developed a cotton bud coated with N-doped carbon dot and chitosan composite in vortex-assisted solid-phase extraction to extract E2, E1, and T before quantitative analysis by high-performance liquid chromatography coupled with a diode array detector (HPLC-DAD). Under optimum HPLC-DAD condition, the linear range for E2, E1, and T were 0.1–1000 mg L⁻¹, 0.05–1000 mgL⁻¹, and 0.1–1000 mgL⁻¹, respectively, with a coefficient of determination (R²) greater than 0.99. In terms of limits of detection (LOD), the values were 40.26 ± 0.11 μg L⁻¹ for E2, 38.41 ± 0.10 μg L⁻¹ for E1, and 34.26 ± 0.07 μg L⁻¹ for T. And the LOQ was 134.2 ± 0.4 μg L⁻¹ for E2, 128.1 ± 0.3 μg L⁻¹ for E1 and 114.2 ± 0.2 μg L⁻¹ for T. This study's developed method can extract spiked E2, E1, and T at a concentration of 75 μg L⁻¹ with a satisfactory extraction efficiency of 75.8 ± 6.9%, 80.2 ± 5.5%, and 72.9 ± 4.6% for E2, E1, and T respectively. As per the result of the experiment, this simple and cheap sorbent can extract steroid hormones, and further optimization of the desorption and extraction parameters is expected to improve recovery.

Copyright

© 2023 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Deki
AU  - Panote Thavarungkul
AU  - Proespichaya Kanatharana
AU  - Chongdee Thammakhet Buranachai
PY  - 2023
DA  - 2023/04/14
TI  - A Cotton Bud Coated with Chitosan/N-Carbon Dot Composite as SPE Sorbent for Extraction of Steroid Hormones from Fish Farm Water
BT  - Proceedings of the 1st Nusa Tenggara International Conference on Chemistry (NITRIC 2022)
PB  - Atlantis Press
SP  - 5
EP  - 12
SN  - 2590-3195
UR  - https://doi.org/10.2991/978-94-6463-130-2_2
DO  - 10.2991/978-94-6463-130-2_2
ID  - 2023
ER  -