Proceedings of the International Conference on Management, Computer and Education Informatization

Anti-tumor Immunity Induced by Heat Shock Protein 72 and Alpha-fetoprotein Epitope Peptide Vaccine

Authors
Yan Fang, Xiaoping Wang, Bing Xu, Penglong Yu
Corresponding Author
Yan Fang
Available Online June 2015.
DOI
https://doi.org/10.2991/mcei-15.2015.56How to use a DOI?
Keywords
Heat shock protien 72 (HSP72); Alpha-fetoprotein (AFP); Vaccine; Tumor; Immunotherapy
Abstract
Objective: Alpha-fetoprotein (AFP) is an oncofetal antigen during hepatocellular carcinoma (HCC) development which could lead to weak reproducible antitumor immunity, and may act as a target for cancer therapy. Therefore, it is imperative to enhance its immunogenicity and develop therapeutic vaccines to eliminate AFP-expressing tumors. The study is tntended to develop a specific anti-tumor peptide vaccine-heat shock protien 72 (HSP72) and alpha-fetoprotein epitope peptide (AFP-P). Methods: By way of glutaraldehyde cross-linking, we constructed a potential therapeutic peptide vaccine, HSP72/AFP-P. In vitro and in vivo cytotoxicity assays were uesd to verify anti-tumor effect of the recombined vaccine. Results: The results showed that cross-linking of AFP epitope peptide with HSP72 induced significant enhancement in specific AFP CD8+ T cells response and distinct cytotoxic antitumor reaction against AFP-expressing tumors. Primimg mice with the reconstructed vaccine, we elicited robust strong protective immunity. Conclusion: Our study suggests that tumor vaccination through cross-linking tumor antigen and HSP72 is an encouraging method for cancer immunotherapy.
Open Access
This is an open access article distributed under the CC BY-NC license.

Download article (PDF)

Proceedings
International Conference on Management, Computer and Education Informatization
Part of series
Advances in Computer Science Research
Publication Date
June 2015
ISBN
978-94-6252-118-6
ISSN
2352-538X
DOI
https://doi.org/10.2991/mcei-15.2015.56How to use a DOI?
Open Access
This is an open access article distributed under the CC BY-NC license.

Cite this article

TY  - CONF
AU  - Yan Fang
AU  - Xiaoping Wang
AU  - Bing Xu
AU  - Penglong Yu
PY  - 2015/06
DA  - 2015/06
TI  - Anti-tumor Immunity Induced by Heat Shock Protein 72 and Alpha-fetoprotein Epitope Peptide Vaccine
BT  - International Conference on Management, Computer and Education Informatization
PB  - Atlantis Press
SN  - 2352-538X
UR  - https://doi.org/10.2991/mcei-15.2015.56
DO  - https://doi.org/10.2991/mcei-15.2015.56
ID  - Fang2015/06
ER  -