Protein Profile of Pangasius (Pangasius hypophthalmus) with Variations Before and After Wet Salting Based on SDS-PAGE

DOI

10.2991/978-94-6463-457-0_9How to use a DOI?

Keywords

Pangasius; Protein Profile; wet salting; SDS-PAGE

Abstract

Pangasius (Pangasius hypophthalmus) is a source of high animal protein and a freshwater commodity that is widely cultivated in Indonesia. One of the disadvantages of pangasius as a food ingredient is that it rots easily. To avoid spoilage, the fish is preserved using wet salting. The purpose of this study was to describe the protein profile of pangasius based on variations in wet salt concentration using the Gel Electrophoresis (SDS-PAGE) method. The samples used were five parts of pangasius which were treated for 12 hours without salting treatment, three parts for wet salting treatment with a salt solution concentration of 10%, 20%, and 30% w/v for 12 hours, and fresh fish meat. The type of research is experimental from profile protein and organoleptic tests. This research was a descriptive analysis by looking at the result of SDS-PAGE. The results showed that the highest concentration of protein is 27,92 µg/µL after 10% b/v of wet salting treatment compared to pangasius before salting treatment. The best protein profile was a concentration of 10% because the protein weights ranging from 249-48 kDa were almost the same as fresh pangasius. The conclusion is the best concentration after wet salting was the concentration of 10% because the protein concentration, organoleptic tests, and protein profile were almost the same as fresh pangasius.

Copyright

© 2024 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Meutia Srikandi Fitria
AU  - Roma Dhona Beauty Zakiya
AU  - Aprilia Indra Kartika
AU  - Aditya Rahman Ernanto
PY  - 2024
DA  - 2024/07/22
TI  - Protein Profile of Pangasius (Pangasius hypophthalmus) with Variations Before and After Wet Salting Based on SDS-PAGE
BT  - Proceedings of the 2nd Lawang Sewu International Symposium on Health Sciences: Medical Laboratory Technology (LSISHS-MLT 2023)
PB  - Atlantis Press
SP  - 75
EP  - 86
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-457-0_9
DO  - 10.2991/978-94-6463-457-0_9
ID  - Fitria2024
ER  -