Bioelectrode based on reconstitution of a dye-linked L-proline dehydrogenase on gold electrode modified by PQQ and FAD
Authors
Yanhui Song, Ningxin Wang, Haitao Zheng, Shin-ichiro Suye
Corresponding Author
Yanhui Song
Available Online October 2015.
- DOI
- 10.2991/iwmecs-15.2015.77How to use a DOI?
- Keywords
- L-proline sensor, reconstitution of apo-enzyme, electron relay.
- Abstract
An electrochemical sensor was developed by reconstituting pyrroloquinoline quinone (PQQ), flavin adenine dinucleotide (FAD) and L-proline dehydrogenase on gold electrode. Reversible redox peaks and low redox potential were obtained from cyclic voltammograms, proved that the attached PQQ and FAD accelerated electron communication between enzyme active site and gold electrode surface. It is expected that this reconstitution technology is suitable for the application of this modified electrode to bioanode.
- Copyright
- © 2015, the Authors. Published by Atlantis Press.
- Open Access
- This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
Cite this article
TY - CONF AU - Yanhui Song AU - Ningxin Wang AU - Haitao Zheng AU - Shin-ichiro Suye PY - 2015/10 DA - 2015/10 TI - Bioelectrode based on reconstitution of a dye-linked L-proline dehydrogenase on gold electrode modified by PQQ and FAD BT - Proceedings of the 2015 2nd International Workshop on Materials Engineering and Computer Sciences PB - Atlantis Press SP - 385 EP - 388 SN - 2352-538X UR - https://doi.org/10.2991/iwmecs-15.2015.77 DO - 10.2991/iwmecs-15.2015.77 ID - Song2015/10 ER -