Bioelectrode based on reconstitution of a dye-linked L-proline dehydrogenase on gold electrode modified by PQQ and FAD

DOI

10.2991/iwmecs-15.2015.77How to use a DOI?

Keywords

L-proline sensor, reconstitution of apo-enzyme, electron relay.

Abstract

An electrochemical sensor was developed by reconstituting pyrroloquinoline quinone (PQQ), flavin adenine dinucleotide (FAD) and L-proline dehydrogenase on gold electrode. Reversible redox peaks and low redox potential were obtained from cyclic voltammograms, proved that the attached PQQ and FAD accelerated electron communication between enzyme active site and gold electrode surface. It is expected that this reconstitution technology is suitable for the application of this modified electrode to bioanode.

Copyright

© 2015, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Yanhui Song
AU  - Ningxin Wang
AU  - Haitao Zheng
AU  - Shin-ichiro Suye
PY  - 2015/10
DA  - 2015/10
TI  - Bioelectrode based on reconstitution of a dye-linked L-proline dehydrogenase on gold electrode modified by PQQ and FAD
BT  - Proceedings of the 2015 2nd International Workshop on Materials Engineering and Computer Sciences
PB  - Atlantis Press
SP  - 385
EP  - 388
SN  - 2352-538X
UR  - https://doi.org/10.2991/iwmecs-15.2015.77
DO  - 10.2991/iwmecs-15.2015.77
ID  - Song2015/10
ER  -