Determining the Polymorphism of V397I SNP of Growth Differentiation Factor 9 (GDF9) gene in Indonesian Saanen Goats

DOI

10.2991/absr.k.220309.032How to use a DOI?

Keywords

GDF9 gene; Reproduction; Saanen goats; T-ARMS-PCR; V397I SNP

Abstract

Reproduction is an economically important trait. It is key to the profitability and sustainability of the goat industry. The Goat industry plays a major role in the Indonesian livestock industry, thus genetic improvement of reproductive traits of goats in Indonesia may be of great benefit to the industry. GDF9 gene is one of the most common and largely studied genes with regards to fecundity in goats. Many GDF9 gene SNPs have been reported and the V397I SNP has been studied extensively about to with fecundity in goats. Therefore, the current study aimed to determine the polymorphism of V397I SNP in Indonesian Saanen goats at Baturraden breeding centre. The study used T-ARMS-PCR for genotyping and the predicted alleles (186 bp for G allele and 275 bp for A allele) were reported. The population was monomorphic for genotype AG which is associated with relatively high litter size. Therefore, the study concluded that the T-ARMS-PCR method can be used successfully to genotype V397I SNP. Although the SNP was monomorphic, the findings of the study revealed that the SNP has the potential to be used as a marker for reproduction performance in indigenous breeds and also non-selected lines. The study also suggested that the polymorphism of the V397I SNP should be determined in larger sample size, various breeding centres or studs and (or) non-selected population of Indonesian goats.

Copyright

© 2022 The Authors. Published by Atlantis Press International B.V.

Open Access

This is an open access article under the CC BY-NC license.

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TY  - CONF
AU  - Janet Mamutse
AU  - Dattadewi Purwantini
AU  - Agus Susanto
AU  - Akhmad Sodiq
PY  - 2022
DA  - 2022/03/29
TI  - Determining the Polymorphism of V397I SNP of Growth Differentiation Factor 9 (GDF9) gene in Indonesian Saanen Goats
BT  - Proceedings of the International Conference on Improving Tropical Animal Production for Food Security (ITAPS 2021)
PB  - Atlantis Press
SP  - 155
EP  - 158
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.220309.032
DO  - 10.2991/absr.k.220309.032
ID  - Mamutse2022
ER  -