Proceedings of the International Conference on Improving Tropical Animal Production for Food Security (ITAPS 2021)

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Effect of the Addition of Egg Yolk to Skim Milk Extender and Storage Time on the Motility and Fertility of Kampung Rooster Spermatozoa

Authors
Dadang Mulyadi Saleh1, *, Mas Yedi Sumaryadi1, Aras Prasetiyo Nugroho1, Chomsiatun Nurul Hidayah1
1Faculty of Animal Science, University of Jenderal Soedirman, Central Java, Indonesia
*Corresponding author. Email: dadang.saleh@unsoed.ac.id
Corresponding Author
Dadang Mulyadi Saleh
Available Online 29 March 2022.
DOI
10.2991/absr.k.220309.021How to use a DOI?
Keywords
egg yolk; skim milk; motility; fertility; Kampung rooster spermatozoa
Abstract

The aim of this study was to evaluate the egg yolk addition and storage time on the motility and fertility of Kampung rooster spermatozoa. A completely randomized design with a 3x2 factorial pattern was used in this study. E factor is the addition of egg yolk into skim milk. (E1 = skim milk + egg yolk 15%, E2 = skim milk + egg yolk 20%, E3 = skim milk + egg yolk 25%) and S factor is storage time (S1 = 60 minutes, S2 = 180 minutes), at 5 °C. Pooled 10 kampung rooster semen was allocated into six experimental groups. Motility assessment in each treatment group was carried out five times. Fertility assessment was obtained by inseminating ten hens of Isa Brown (n=60) for each treatment for seven days egg collection. There is no interaction effect between storage time and egg yolk addition (P>0.05) on the motility and fertility of kampung rooster sperm. The mean percentage motility and fertility of kampung rooster sperm in extender containing different egg yolk were (72.5±1.73; 67.5±1.73; 65.83±1.81%, P>0.05), and (94.13±9.35; 88.64±8.97 and 91.61±11.26; P>0.05), respectively. The average sperm motility and Fertility in a different storage time were (70.83±3.53% and 67.78±3.47%, P>0.05) and (91.06±5.77% and 91.86±3.26%, P>0.05), respectively. It can be concluded that the addition of 15, 20 and 25 % egg yolk to skim milk stored at 5°C refrigerator temperature for up to three hours can be used to dilute Kampung rooster semen.

Copyright
© 2022 The Authors. Published by Atlantis Press International B.V.
Open Access
This is an open access article under the CC BY-NC license.

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Volume Title
Proceedings of the International Conference on Improving Tropical Animal Production for Food Security (ITAPS 2021)
Series
Advances in Biological Sciences Research
Publication Date
29 March 2022
ISBN
978-94-6239-557-2
ISSN
2468-5747
DOI
10.2991/absr.k.220309.021How to use a DOI?
Copyright
© 2022 The Authors. Published by Atlantis Press International B.V.
Open Access
This is an open access article under the CC BY-NC license.

Cite this article

risenwbib
TY  - CONF
AU  - Dadang Mulyadi Saleh
AU  - Mas Yedi Sumaryadi
AU  - Aras Prasetiyo Nugroho
AU  - Chomsiatun Nurul Hidayah
PY  - 2022
DA  - 2022/03/29
TI  - Effect of the Addition of Egg Yolk to Skim Milk Extender and Storage Time on the Motility and Fertility of Kampung Rooster Spermatozoa
BT  - Proceedings of the International Conference on Improving Tropical Animal Production for Food Security (ITAPS 2021)
PB  - Atlantis Press
SP  - 101
EP  - 104
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.220309.021
DO  - 10.2991/absr.k.220309.021
ID  - Saleh2022
ER  -