Characteristics of Ruminal Fatty Acids Using In Vitro Culture System by Addition of Galangal (Alpinia galangal) Essential oil

DOI

10.2991/absr.k.220207.014How to use a DOI?

Keywords

Galangal essential oils; poly unsaturated fatty acids; cineole; in vitro rumen fermentatio

Abstract

Galangal essential oil (EO), which contains secondary metabolites such as cineol, hopefully can modify bacteria, causing biohydrogenation. The purpose of this study was to determine the effect of galangal EO dose on the fatty acids characteristics at in vitro rumen fermentation. Galangal as a source of EO was obtained from the local market and then extracted by steam distillation method. The phytochemical compounds of galangal were evaluated using gas chromatography-mass spectrophotometry. Each treatment consisted of the same basal feed (60% elephant grass and 40% concentrate) with varying doses of galangal EO as much as 0 (control), 30, 60, 120 µL and pure cineol as much as 5µL. After 48 hours of in vitro rumen fermentation, the fermated media was harvested for its fatty acid analysis by gas chromatography. The data obtained were analyzed for variance using a completely randomized design with five treatments and six replications. The concentration of MUFA (oleic acid) increased significantly by 22.16%, 43.09%, and 42.19% compared to control at.60 and 120 µL doses of EO galangal, respectively. PUFA concentrations did not significant (P>0.05) between galangal EO at doses of 0 (control) and 30 µL and decreased significantly (P<0.05) at doses of 60 and 120 µL. The addition of EO galangal 60 µL showed higher oleic acid concentration than 5 µL pure cineol, although both treatmens has the same cineol content. The addition of 5 µL of pure cineol showed that the PUFA concentration did not significant (P>0.05) from the control (without galangal EO). This study concluded that galangal essential oil increased unsaturated fatty acids levels with the optimum dosage of 60 µL

Copyright

© 2022 The Authors. Published by Atlantis Press International B.V.

Open Access

This is an open access article under the CC BY-NC license.

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TY  - CONF
AU  - Dewi Ratih Ayu Daning
AU  - Chusnul Hanim
AU  - Budi Prasetyo Widyobroto
AU  - Lies Mira Yusiati
PY  - 2022
DA  - 2022/02/24
TI  - Characteristics of Ruminal Fatty Acids Using In Vitro Culture System by Addition of Galangal (Alpinia galangal) Essential oil
BT  - Proceedings of the 9th International Seminar on Tropical Animal Production (ISTAP 2021)
PB  - Atlantis Press
SP  - 65
EP  - 69
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.220207.014
DO  - 10.2991/absr.k.220207.014
ID  - Daning2022
ER  -