Proceedings of the 2022 6th International Seminar on Education, Management and Social Sciences (ISEMSS 2022)

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CRISPR-Cas9 Fundamental Uses: Analysis of Human Genome Engineering Through CRISPR/Cas9

Authors
Yushun Tian1, *
1Walt Whitman High School, Bethesda, MD, USA
*Corresponding author. Email: 167922@mcpsmd.net
Corresponding Author
Yushun Tian
Available Online 29 December 2022.
DOI
10.2991/978-2-494069-31-2_289How to use a DOI?
Keywords
CRISPR/Cas9; gene editing; therapeutics; guide RNA; bioethics
Abstract

The invention of CRISPR-Cas9 allowed the wanted genome to be targeted and edited. Clustered regularly interspaced short palindromic repeats (CRISPR) combined with Cas9 protein together can be diverted to a guided location in the genome, editing the DNA strand. This achievement improved the human body’s genomes and turned them into cells. CRISPR-Cas9 could be used in cells and animal model generations, and it has been proven that human embryos can be similarly modified. The passage analysis has also included the type II bacterial CRISPR system with custom guide RNA (gRNA) in human cells, which allowed the team to implement sophisticated investigations and experiments: endogenous AAVS1 locus in targeting T cells, K562 cells, and induced pluripotent stem cells. The experiment research provides guidelines for the future development target sites, cleavage efficiency, and off-target activity analysis.

Copyright
© 2022 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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Volume Title
Proceedings of the 2022 6th International Seminar on Education, Management and Social Sciences (ISEMSS 2022)
Series
Advances in Social Science, Education and Humanities Research
Publication Date
29 December 2022
ISBN
10.2991/978-2-494069-31-2_289
ISSN
2352-5398
DOI
10.2991/978-2-494069-31-2_289How to use a DOI?
Copyright
© 2022 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

Cite this article

risenwbib
TY  - CONF
AU  - Yushun Tian
PY  - 2022
DA  - 2022/12/29
TI  - CRISPR-Cas9 Fundamental Uses: Analysis of Human Genome Engineering Through CRISPR/Cas9
BT  - Proceedings of the 2022 6th International Seminar on Education, Management and Social Sciences (ISEMSS 2022)
PB  - Atlantis Press
SP  - 2464
EP  - 2473
SN  - 2352-5398
UR  - https://doi.org/10.2991/978-2-494069-31-2_289
DO  - 10.2991/978-2-494069-31-2_289
ID  - Tian2022
ER  -