DNA barcoding of an amphidromous palaemonid shrimp recruiting to the Palu River, Central Sulawesi, Indonesia

DOI

10.2991/978-94-6463-334-4_26How to use a DOI?

Keywords

—Macrobrachium australe; Macrobrachium indicum; COI mitochondrial DNA; Palaemonidae; Wallacea

Abstract

Sulawesi is the largest island in the Wallacea biodiversity hotspot, but many aspects of this biodiversity are still poorly known, including diadromous fish and invertebrates. As part of a research program to identify the taxa migrating upstream in the Palu River estuary together with multispecies schools of anguillid glass eels, sampling during the August 2022 dark moon period yielded glass eels, other fish, mollusks, and crustaceans (shrimp and crabs). This study used molecular methods to identify the shrimp collected. DNA barcoding targeted a segment of the Cytochrome Oxidase I Mitochondrial DNA (COI mtDNA) molecular marker (Sanger sequencing of PCR product) and analysis was implemented in MEGA 11. The NCBI BLAST-n online routine was used to obtain homologous nucleotide accessions similar to the 612 bp Palu crustacean barcode. Matches with 100% identity submitted as Macrobrachium australe ranged from 91–100% coverage (Accessions MN526188-MN526195) while matches with 100% identity submitted as M. indicum had 85% coverage (Accessions KX866570- KX866577, KX866580 KX866582 and KX866585). Phylogenic analysis (Maximum Likelihood) showed the Palu crustacean nested in the genus Macrobrachium consistent with identification based on external morphology, within an M. australe and M. indicus clade, deeply divided from the clade containing M. rosenbergii.

Copyright

© 2023 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Eka Rosyida
AU  - Abigail Mary Moore
AU  - Novalina Serdiati
AU  - Samliok Ndobe
AU  - Judistira Siddik
PY  - 2024
DA  - 2024/01/24
TI  - DNA barcoding of an amphidromous palaemonid shrimp recruiting to the Palu River, Central Sulawesi, Indonesia
BT  - Proceedings of the 2nd International Interdisciplinary Conference on Environmental Sciences and Sustainable Developments 2022 Environment and Sustainable Development (IICESSD-ESD 2022)
PB  - Atlantis Press
SP  - 153
EP  - 158
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-334-4_26
DO  - 10.2991/978-94-6463-334-4_26
ID  - Rosyida2024
ER  -