Tortoise shell and falsify random amplified polymorphism DNA (RAPD) appraisal method research

DOI

10.2991/ifeesm-15.2015.188How to use a DOI?

Keywords

Tortoise shell; PCR; RAPD; Fingerprint.

Abstract

Purpose To establish a practical, fast and accurate identification method of tortoise shell, falsify amplified polymorphism DNA (RAPD) randomly and provide reliable basis for identification of tortoise shell. Method We use the Alkali cracking method to extract mitochondrial DNA from the tortoise shell and falsify and randomly do the amplification polymorphic DNA. Result The electrocardiogram of genuine and counterfeit goods tortoise shells mitochondrial DNA random amplified has obvious difference, the stripe number and location are all different, this method can effectively distinguish between genuine and counterfeit goods tortoise shells. Conclusion RAPD technology provides a simple, effective, high reliability for the identification of tortoise shell and its adulterants.

Copyright

© 2015, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Zi-tong Li
AU  - Guang-xin Yuan
AU  - Miao Wang
AU  - Li-jun Gao
AU  - Li-hua Zhang
AU  - Ming-cheng Li
AU  - Jia-mu Niu
PY  - 2015/09
DA  - 2015/09
TI  - Tortoise shell and falsify random amplified polymorphism DNA (RAPD) appraisal method research
BT  - Proceedings of the 2015 International Forum on Energy, Environment Science and Materials
PB  - Atlantis Press
SP  - 1019
EP  - 1024
SN  - 2352-5401
UR  - https://doi.org/10.2991/ifeesm-15.2015.188
DO  - 10.2991/ifeesm-15.2015.188
ID  - Li2015/09
ER  -