Proceedings of the 2nd International Conference on Veterinary, Animal, and Environmental Sciences (ICVAES 2020)

Profile of Antigen Excretory-Secretory Schistosoma Japonicum in the Development of Elisa Method to Detect Schistosomiasis in Indonesia

Authors
Samarang, Made Agus Nurjana, Malonda Maksud, Sri Murtini, Fadjar Satrija
Corresponding Author
Samarang
Available Online 21 April 2021.
DOI
10.2991/absr.k.210420.005How to use a DOI?
Keywords
Antigen Excretory-Secretory, Schistosoma japonicum, ELISA
Abstract

The development of ELISA method to detect patients with schistosomiasis in Indonesia is a detection of the antigens that are derived from the peripheral vessels in the blood serum. This research is conducted in the Napu Plateau of Poso Regency, Central Sulawesi, Indonesia. The goal is to develop ELISA method with polyclonal antibodies to detect antigenic-excretory antigen of S. japonicum in patients with schistosomiasis. Activities in the study include activities in the field namely the isolation of worm S. japonicum and activities in laboratories such as production, characterization and purification by Bradford methods as well as electrophoresis test to see the protein Antigen profile. The results of the study of the 30 worm Schistosoma collected from 5 rats suffered schistosomiasis, obtained as much as 42.5 ml with a protein concentration of 1.351 mg/ml, and had two patterns of polypeptide tape with a molecular weight range of 20 and 39 Dalton kilo (kDa). The conclusion of the resulting Antigen ES S. japonicum profile can induce a good polyclonal antibody in the development of ELISA method to detect the AgES in the serum of schistosomiasis.

Copyright
© 2021, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
Proceedings of the 2nd International Conference on Veterinary, Animal, and Environmental Sciences (ICVAES 2020)
Series
Advances in Biological Sciences Research
Publication Date
21 April 2021
ISBN
978-94-6239-367-7
ISSN
2468-5747
DOI
10.2991/absr.k.210420.005How to use a DOI?
Copyright
© 2021, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

TY  - CONF
AU  - Samarang
AU  - Made Agus Nurjana
AU  - Malonda Maksud
AU  - Sri Murtini
AU  - Fadjar Satrija
PY  - 2021
DA  - 2021/04/21
TI  - Profile of Antigen Excretory-Secretory Schistosoma Japonicum in the Development of Elisa Method to Detect Schistosomiasis in Indonesia
BT  - Proceedings of the 2nd International Conference on Veterinary, Animal, and Environmental Sciences (ICVAES 2020)
PB  - Atlantis Press
SP  - 20
EP  - 24
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.210420.005
DO  - 10.2991/absr.k.210420.005
ID  - 2021
ER  -