Classical Enterotoxin Genes of Staphylococcus aureus Isolated from the Raw Milk of Cows and Goats in Yogyakarta Indonesia

DOI

10.2991/absr.k.210420.004How to use a DOI?

Keywords

Staphylococcus aureus, enterotoxin gene, dairy raw milk, multiplex PCR

Abstract

Milk is a highly nutritious food containing several essential nutrients. Consequently, it can be contaminated by pathogenic bacteria, including Staphylococcus aureus. This study aimed to analyze the genes encoding nine classical enterotoxins of S. aureus isolated from cow and goat raw milk. A total of 75 S. aureus isolates were obtained from milk samples of dairy cows (46 isolates) and Peranakan Etawa (PE) goats (29 isolates) from different farms in Yogyakarta Indonesia. Based on the cultural and biochemical properties and the 23S rRNA occurrence, all isolates could be identified as S. aureus. The seh gene was the most frequently observed in 21 isolates (45.65%) of cows, followed by the sea gene (in 8 isolates, 17.39%), the seg and the sei genes (each in 4 isolates, 8.69%). The sec is the only staphylococcal enterotoxin gene observed in S. aureus isolated from goat milk with the prevalence of 6.89%. The genes encoding four out of nine classical enterotoxins (seh, sea, seg, and sei) are commonly isolated from S. aureus in cow milk, but only sec gene can be found in the isolates from goat milk.

Copyright

© 2021, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Desy Cahya Widianingrum
AU  - Sarasati Windria
AU  - Fatkhanuddin Aziz
AU  - Siti Isrina Oktavia Salasia
PY  - 2021
DA  - 2021/04/21
TI  - Classical Enterotoxin Genes of Staphylococcus aureus Isolated from the Raw Milk of Cows and Goats in Yogyakarta Indonesia
BT  - Proceedings of the 2nd International Conference on Veterinary, Animal, and Environmental Sciences (ICVAES 2020)
PB  - Atlantis Press
SP  - 15
EP  - 19
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.210420.004
DO  - 10.2991/absr.k.210420.004
ID  - Widianingrum2021
ER  -