The Effect of L-Ascorbic Acid-2-Phosphate on Human Adipose-Derived Stem Cells Population Doubling Time and Angiogenic Potency

DOI

10.2991/978-94-6463-180-7_42How to use a DOI?

Keywords

ADSCs; L-ascorbic acid-2-phosphate; PDT; angiogenic potency

Abstract

Adipose-derived stem cells (ADSCs) have been developed as substitute cells for tissue engineering. However, in order to get a high number of cells, over-repassage leads the cells to become big in spread size indicating that senescence occurred. This study aimed to evaluate the potency of ascorbic acid to induce the proliferation and angiogenic potency of human ADSCs and inhibit the aging process of the cells. Human ADSCs were isolated by an enzymatic process. ADSCs were cultured in 10% fetal bovine serum (FBS) supplemented by ascorbic acid in various concentrations (1, 3, 6 µg/ml). The morphology, proliferation, population doubling time (PDT), and angiogenic potency were evaluated. Angiogenic potency analysis was performed using gene expression of vascular endothelial growth factor (VEGF) and its receptor (VEGFR). Ascorbic acid on 6 µg/mL suppressed the senescence proved by cell spread areas, growth curves, viability, PDT, and percentage of scratch width closure. No significant difference in vascular endothelial growth factor and its receptor expression to the control. However, the high concentration of ascorbic acid (6 µg/ml) expressed the highest VEGF and VEGFR. Our study showed that ADSCs with supplementation of ascorbic acid (6 µg/ml) promoted proliferation and angiogenic potency.

Copyright

© 2023 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Imam Rosadi
AU  - Karina Karina
AU  - Viol Dhea Kharisma
AU  - Arif Nur Muhammad Ansori
PY  - 2023
DA  - 2023/07/27
TI  - The Effect of L-Ascorbic Acid-2-Phosphate on Human Adipose-Derived Stem Cells Population Doubling Time and Angiogenic Potency
BT  - Proceedings of the International Conference of Tropical Studies and its Applications (ICTROPS 2022)
PB  - Atlantis Press
SP  - 401
EP  - 408
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-180-7_42
DO  - 10.2991/978-94-6463-180-7_42
ID  - Rosadi2023
ER  -