Induction of Polyploidy Using Colchicine in Flower Buds from Phalaenopsis Hybrids

Sri Hartati; Samanhudi; Ongko Cahyono; Agung Wibowo; Hasna Afanin

doi:10.2991/978-94-6463-128-9_41

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Induction of Polyploidy Using Colchicine in Flower Buds from Phalaenopsis Hybrids

Authors

Sri Hartati¹^{, 2}^{, *}, Samanhudi¹^{, 2}, Ongko Cahyono³, Agung Wibowo⁴, Hasna Afanin¹

¹Department of Agrotechnology, Faculty of Agriculture, Sebelas Maret University, Jl. Ir. Sutami 36A, Surakarta, 57126, Central Java, Indonesia

²Center for Biotechnology and Biodiversity Research and Development, Institute of Research and Community Services, Sebelas Maret University, Jl. Ir. Sutami 36A Kentingan Jebres, Surakarta, 57126, Indonesia

³Department of Soil Science, Faculty of Agriculture, Sebelas Maret University, Jl. Ir. Sutami 36A, Surakarta, 57126, Central Java, Indonesia

⁴Department of Agricultural Extension and Communication, Faculty of Agriculture, Sebelas Maret University, Jl. Ir. Sutami 36A, Surakarta, 57126, Central Java, Indonesia

^*Corresponding author. Email: tatik_oc@yahoo.com

Corresponding Author

Sri Hartati

Available Online 19 April 2023.

DOI: 10.2991/978-94-6463-128-9_41 How to use a DOI?
Keywords: orchid; chromosome; karyotype
Abstract: Phalaenopsis hybrid is one of the orchids that is in great demand in the horticultural market as cut and potted flowers. One of the attempt to obtain new variation flower characters in Phalaenopsis hybrids is polyploidization using colchicine. The purpose of this study was to determine the cytological character of orchids (number, size, shape of chromosomes). The research was conducted at the Screenhouse of the Faculty of Agriculture and Integrated Laboratory Unit of Sebelas Maret University, Surakarta in August 2021-March 2022. Polyploidy induction was carried by dripping 1500 ppm concentration of colchicine on flower bud of Phalaenopsis hybrid. The method of this research using squash preparation for chromosome analysis. The results of this research showed that number of chromosomes in control Phalaenopsis Golden Tree 2n = 3x − 7 = 50, Phalaenopsis Fuller Sunset and Phalaenopsis OX X-ray 2n = 4x = 76. Phalaenopsis Golden Tree which were treated with 1500 ppm colchicine each underwent polyploidization with number of chromosomes 2n = 6x − 14 = 100, Phalaenopsis Fuller Sunset and Phalaenopsis OX X-ray 2n = 8x = 152. Phalaenopsis Golden Tree, Phalaenopsis Fuller Sunset and Phalaenopsis OX X-ray has a relatively small chromosome size, and has the same chromosome shape, namely metacentric.
Open Access: Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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Volume Title: Proceedings of the 3rd International Conference on Sustainable Agriculture for Rural Development (ICSARD 2022)
Series: Advances in Biological Sciences Research
Publication Date: 19 April 2023
ISBN: 978-94-6463-128-9
ISSN: 2468-5747
DOI: 10.2991/978-94-6463-128-9_41 How to use a DOI?
Open Access: Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

Cite this article

ris enw bib

TY  - CONF
AU  - Sri Hartati
AU  - Samanhudi
AU  - Ongko Cahyono
AU  - Agung Wibowo
AU  - Hasna Afanin
PY  - 2023
DA  - 2023/04/19
TI  - Induction of Polyploidy Using Colchicine in Flower Buds from Phalaenopsis Hybrids
BT  - Proceedings of the 3rd International Conference on Sustainable Agriculture for Rural Development (ICSARD 2022)
PB  - Atlantis Press
SP  - 423
EP  - 430
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-128-9_41
DO  - 10.2991/978-94-6463-128-9_41
ID  - Hartati2023
ER  -

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