Toxicity Test of Column Chromatography Steroids Isolates from Ethyl Acetate Fraction of Hydrilla verticillata

DOI

10.2991/assehr.k.210421.050How to use a DOI?

Keywords

Toxicity, Column Chromatography, Steroid, Ethyl acetate, Hydrilla verticillata

Abstract

Hydrilla verticillata is one of the aquatic plants that contain some secondary metabolites, such as triterphenoids and steroids. The aim of this study was to determine the toxicity levels of steroids isolated from ethyl acetate fraction of Hydrilla verticillata separated by column chromatoghraphy. The biomass of Hydrilla verticillata was dried and powdered. Hydrilla verticillata powder is then extracted by maceration methods using ethanol solvent. The crude ethanol extracts were hydrolyzed using 2 N of HCl and partitioned using ethyl acetate solvent. The steroids compounds of ethyl acetate fraction were separated by Column Chromatography. The steroid isolates from Column Chromatography were identified by FTIR and UV-Vis spectrophotometer. The toxicity level of Column Chromatography steroid isolates was determined by BSLT method. The result of this research showed that extraction through maceration using ethanol produced 2.52 % yield, whereas the percent yield of the partition using ethyl acetate was 45.04 %. Separation by column chromatography resulted 3 steroids isolates B1, B2, and G1. The toxicity test showed that column chromatography steroids isolate from ethyl acetate fraction of Hydrilla verticillata has toxicity properties. The LC50 value of B1, B2 and G1 isolate was 14.58, 7.55 and 4.71 ppm, respectively.

Copyright

© 2021, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - A Ghanaim Fasya
AU  - Suci Amalia
AU  - Dewi Sinta Megawati
AU  - Vivi Septya Wati
AU  - Fitri Fatimah
AU  - Hasan Ali Mahbubi
AU  - Vera Vania
PY  - 2021
DA  - 2021/04/22
TI  - Toxicity Test of Column Chromatography Steroids Isolates from Ethyl Acetate Fraction of Hydrilla verticillata
BT  - Proceedings of the International Conference on Engineering, Technology and Social Science (ICONETOS 2020)
PB  - Atlantis Press
SP  - 352
EP  - 356
SN  - 2352-5398
UR  - https://doi.org/10.2991/assehr.k.210421.050
DO  - 10.2991/assehr.k.210421.050
ID  - Fasya2021
ER  -