Molecular Identification Beta Hemolysis Isolates Species from Contact Lens Cleaner Residual Solution
- DOI
- 10.2991/978-94-6463-018-3_5How to use a DOI?
- Keywords
- Contact Lens; Beta hemoliysis; Molecular Identification
- Abstract
A contact lens cleaner solution should not be left in place for storage and reuse for subsequent storage. The cleaner that has been used does not have enough disinfectant power, even becomes a place of microorganism growth so the risk of infection increases. The study aims to identify the bacterial isolates of the beta hemolysis contained in the residual cleaning fluid the lens contact. The sample in this research was taken from all students in the STIKES Mandala Waluya Kendari who wear contact lenses as many as 10 people. The suspension of bacteria obtained is then planted on media BA (Blood agar) with the streak plate method. Isolates showing beta hemolysis then identified using amplification gene 16S rRNA using primers universal primers 27 F/1495 R, the results of the amplification electrophoresis with agarose of 1.5% and subsequent sequencing on the gene 16S rRNA. Based on the results of this study show the 10 isolates, namely W1, W2, W3, W4, W5, W6, W7, W8, W9, and W10 showed positive results which are characterized by the growth of bacteria on BA medium and there is one isolate of beta hemolysis namely sample W6. Molecular identification has shown that isolates W6 shared a 93% percent identity with the species Bacillus sp.
- Copyright
- © 2023 The Author(s)
- Open Access
- Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.
Cite this article
TY - CONF AU - Sri Anggarini Rasyid AU - Sanatang Sanatang AU - Widya Ningsih AU - Muzuni Muzuni AU - Satriani Syarif AU - Sugireng Sugireng PY - 2022 DA - 2022/12/15 TI - Molecular Identification Beta Hemolysis Isolates Species from Contact Lens Cleaner Residual Solution BT - Proceedings of the First International Conference on Medical Technology (ICoMTech 2021) PB - Atlantis Press SP - 34 EP - 42 SN - 2468-5739 UR - https://doi.org/10.2991/978-94-6463-018-3_5 DO - 10.2991/978-94-6463-018-3_5 ID - Rasyid2022 ER -