Proceedings of the First International Conference on Medical Technology (ICoMTech 2021)

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Molecular Identification Beta Hemolysis Isolates Species from Contact Lens Cleaner Residual Solution

Authors
Sri Anggarini Rasyid1, *, Sanatang Sanatang1, Widya Ningsih1, Muzuni Muzuni2, Satriani Syarif1, Sugireng Sugireng1
1D-IV Medical Laboratory Technology Study Program, Mandala Waluya University, Kendari, Southeast Sulawesi, Indonesia
2Department of Biology, Faculty of Mathematics and Natural Sciences, Halu Oleo University, Kendari, Southeast Sulawesi, Indonesia
*Corresponding author. Email: anggarini.09@gmail.com
Corresponding Author
Sri Anggarini Rasyid
Available Online 15 December 2022.
DOI
10.2991/978-94-6463-018-3_5How to use a DOI?
Keywords
Contact Lens; Beta hemoliysis; Molecular Identification
Abstract

A contact lens cleaner solution should not be left in place for storage and reuse for subsequent storage. The cleaner that has been used does not have enough disinfectant power, even becomes a place of microorganism growth so the risk of infection increases. The study aims to identify the bacterial isolates of the beta hemolysis contained in the residual cleaning fluid the lens contact. The sample in this research was taken from all students in the STIKES Mandala Waluya Kendari who wear contact lenses as many as 10 people. The suspension of bacteria obtained is then planted on media BA (Blood agar) with the streak plate method. Isolates showing beta hemolysis then identified using amplification gene 16S rRNA using primers universal primers 27 F/1495 R, the results of the amplification electrophoresis with agarose of 1.5% and subsequent sequencing on the gene 16S rRNA. Based on the results of this study show the 10 isolates, namely W1, W2, W3, W4, W5, W6, W7, W8, W9, and W10 showed positive results which are characterized by the growth of bacteria on BA medium and there is one isolate of beta hemolysis namely sample W6. Molecular identification has shown that isolates W6 shared a 93% percent identity with the species Bacillus sp.

Copyright
© 2023 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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Volume Title
Proceedings of the First International Conference on Medical Technology (ICoMTech 2021)
Series
Advances in Health Sciences Research
Publication Date
15 December 2022
ISBN
10.2991/978-94-6463-018-3_5
ISSN
2468-5739
DOI
10.2991/978-94-6463-018-3_5How to use a DOI?
Copyright
© 2023 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

Cite this article

risenwbib
TY  - CONF
AU  - Sri Anggarini Rasyid
AU  - Sanatang Sanatang
AU  - Widya Ningsih
AU  - Muzuni Muzuni
AU  - Satriani Syarif
AU  - Sugireng Sugireng
PY  - 2022
DA  - 2022/12/15
TI  - Molecular Identification Beta Hemolysis Isolates Species from Contact Lens Cleaner Residual Solution
BT  - Proceedings of the First International Conference on Medical Technology (ICoMTech 2021)
PB  - Atlantis Press
SP  - 34
EP  - 42
SN  - 2468-5739
UR  - https://doi.org/10.2991/978-94-6463-018-3_5
DO  - 10.2991/978-94-6463-018-3_5
ID  - Rasyid2022
ER  -