Proceedings of the International Conference on Medical Science and Health (ICOMESH 2024)

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Detection of Non-coding RNA (hsa_circ_0003416) in Pulmonary Arterial Hypertension Patients’ Plasma by qRT-PCR Analysis

Authors
Fajri Marindra Siregar1, 2, Dicka Wahyu Setiasari3, Sofia Mubarika Haryana4, Dyah Wulan Anggrahini5, Lucia Kris Dinarti5, Anggoro Budi Hartopo5, *
1Doctorate Program, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia
2Department of Biochemistry, Faculty of Medicine, Universitas Riau, Pekanbaru, Indonesia
3Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia
4Department of Histology and Cell Biology, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia
5Department of Cardiology and Vascular Medicine, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada–Dr. Sardjito Hospital, Yogyakarta, Indonesia
*Corresponding author. Email: a_bhartopo@ugm.ac.id
Corresponding Author
Anggoro Budi Hartopo
Available Online 19 December 2024.
DOI
10.2991/978-94-6463-604-8_17How to use a DOI?
Keywords
Circular RNA; hsa_circ_0003416; Pulmonary arterial hypertension; Non-coding RNA; qRT-PCR
Abstract

Circular RNA refers to a type of non-coding RNA molecule and possesses a circular conformation. Circular RNA has greater stability compared to linear RNA due to its resistance to RNAse activity. Hsa_circ_0003416 is a circular RNA reported to have potential as a biomarker in patients with pulmonary arterial hypertension (PAH). This study aims to detect hsa_circ_0003416 using qRT-PCR in the plasma of PAH patients. The sample was taken from RSUP Dr. Sardjito Yogyakarta. The miRNeasy Serum/Plasma Advanced kit was used to isolate total RNA. Following that, cDNA was generated with an Applied Biosystems thermal cycler and the ExcelRT™ Reverse Transcription Kit II reagent, and qPCR was performed with an Applied Biosystems™ 7500 Real-Time PCR equipment and the SensiFAST™ SYBR® Lo-ROX Kit reagent. Specific divergent primers were utilized. Melting curve analysis and visualization of the amplified products were performed using gel electrophoresis. The qRT-PCR technique achieved a single amplification, resulting in a melting curve value observed at 81.8 ℃. Examination by visualizing the gel electrophoresis results showed a single band that matched the target size, specifically 124 base pairs. In conclusion, the qRT-PCR analysis successfully identified the presence of hsa_circ_0003416 in the plasma of PAH patients.

Copyright
© 2024 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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Volume Title
Proceedings of the International Conference on Medical Science and Health (ICOMESH 2024)
Series
Advances in Health Sciences Research
Publication Date
19 December 2024
ISBN
978-94-6463-604-8
ISSN
2468-5739
DOI
10.2991/978-94-6463-604-8_17How to use a DOI?
Copyright
© 2024 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

Cite this article

risenwbib
TY  - CONF
AU  - Fajri Marindra Siregar
AU  - Dicka Wahyu Setiasari
AU  - Sofia Mubarika Haryana
AU  - Dyah Wulan Anggrahini
AU  - Lucia Kris Dinarti
AU  - Anggoro Budi Hartopo
PY  - 2024
DA  - 2024/12/19
TI  - Detection of Non-coding RNA (hsa_circ_0003416) in Pulmonary Arterial Hypertension Patients’ Plasma by qRT-PCR Analysis
BT  - Proceedings of the International Conference on Medical Science and Health (ICOMESH 2024)
PB  - Atlantis Press
SP  - 190
EP  - 196
SN  - 2468-5739
UR  - https://doi.org/10.2991/978-94-6463-604-8_17
DO  - 10.2991/978-94-6463-604-8_17
ID  - Siregar2024
ER  -