Lipase Production of Aspergillus aculeatus MS. 11 Using Solid State Fermentation on Rubber Seed Press Cake

DOI

10.2991/978-94-6463-062-6_4How to use a DOI?

Keywords

Glucose; Rubber seed; SSF

Abstract

Lipase is an enzyme that has various use in industrial sector. However, the enzyme production cost is considerably high mainly due to expensive fermentation substrate. Therefore, it is necessary to develop effective media formulation to increase production of lipase enzyme without reducing the amount of production. This research aims to know the effectiveness of rubber seed press cake as a fermentation substrate for Aspergillus aculeatus Ms. 11. We observed the effect of glucose and olive oil as a carbon source addition on the substrate, determined the growth profile and lipase production on the substrate in lipase production. Isolates of Ms.11 was inoculated in 14,28 mL MGM medium with substrate variation rubber seed press cake, rubber seed press cake + glucose, rubber seed press cake + olive oil, and combination substrate (rubber seed press cake + glucose + olive oil) during 0, 2, 4, 7 days. The highest lipase activity and lipase productivity in this study shown at 19.23 U/mL and 186.51 Yield/day, there were obtained from rubber seed press cake + olive oil on day 2, whereas the highest specific activity of lipase enzyme (1.16 U/mg) was obtained in substrate variation rubber seed press cake + olive oil on day 7. We concluded that maximum lipase production of Ms. 11 can be obtained using rubber seed press cake with addition of olive oil.

Copyright

© 2023 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Maya Nurjanah
AU  - Miftahul Ilmi
PY  - 2022
DA  - 2022/12/22
TI  - Lipase Production of Aspergillus aculeatus MS. 11 Using Solid State Fermentation on Rubber Seed Press Cake
BT  - Proceedings of the 4th International Conference on Life Sciences and Biotechnology (ICOLIB 2021)
PB  - Atlantis Press
SP  - 24
EP  - 43
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-062-6_4
DO  - 10.2991/978-94-6463-062-6_4
ID  - Nurjanah2022
ER  -