DNA Barcoding of Vanda tricolor Lindl. Based on matK, rbcL and ITS2 Sequences

DOI

10.2991/978-94-6463-062-6_61How to use a DOI?

Keywords

Molecular identification; Specific marker; Vanda orchids

Abstract

Vanda tricolor Lindl. is one of the important ornamental plants favored by many people in the world. However, this plant required many years for vegetative growth before the first flowers emerge. Since the flower is one of the most important organs in morphological identification, an alternative approach through DNA barcoding is one of the best breaking approaches for fast and accurate identification regardless of floral organs availabilty. In this study, three different primer sets (matK, rbcL, ITS2) were used for molecular identification. DNA isolation was carried out using plant genomic isolation kit and was applied as template in PCR reaction. After confirmation using agarose gel electrophoresis and visualized under UV transilluminator, the PCR products were then purified and subjected for sequencing analysis. The sequences length obtained were 408 bp, 317 bp and 461 bp for matK, rbcL, and ITS2, respectively. Further analysis using BLAST and phylogenetic tree construction revealed that all three primer sets are applicable for identification of Vanda orchids. Notably, the ITS2 sequence showed specificity and able to discriminate up to the species level, thus recommended to be used in molecular identification of Vanda orchids.

Copyright

© 2023 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Mukhamad Su’udi
AU  - Waki’atil Rosida
AU  - El Shania Ravitamala
AU  - Dwi Setyati
PY  - 2022
DA  - 2022/12/22
TI  - DNA Barcoding of Vanda tricolor Lindl. Based on matK, rbcL and ITS2 Sequences
BT  - Proceedings of the 4th International Conference on Life Sciences and Biotechnology (ICOLIB 2021)
PB  - Atlantis Press
SP  - 596
EP  - 604
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-062-6_61
DO  - 10.2991/978-94-6463-062-6_61
ID  - Su’udi2022
ER  -