Proceedings of the 4th International Conference on Mechatronics, Materials, Chemistry and Computer Engineering 2015

The study of carbon monoxide placenta blood hemoglobin oxygenation

Authors
Chen Chen, Xiang Wang, Pei Wang, Donglai Qi
Corresponding Author
Chen Chen
Available Online December 2015.
DOI
https://doi.org/10.2991/icmmcce-15.2015.408How to use a DOI?
Keywords
hemoglobin, RBC, HbCO, HbO2.
Abstract

With the great progress of hemoglobin-based blood substitutes, however, they all have a same problem that is hard to get effective stable structure of hemoglobin. The hemoglobin can be oxidized into met hemoglobin in the process of purification, lead to the loss of the function of carrying and transporting oxygen. The combination of CO and placenta blood hemoglobin ability is far higher than that of O2, the structure and properties of carbon monoxide hemoglobin (HbCO) has more stable. Red blood cell (RBC) is mixed with organic solvent for hemolysis and centrifuged for removal of stroma. To prevent MetHb formation during the procedure, Hb was carbonylated in advance. Excessive carbon monoxide pumped to hemoglobin, hemoglobin is converted into HbCO. HbCO is decarbonylated to regenerate HbO2 by exposing the solution to oxygen and visible light, the content of HbCO and HbCO is measured by blood gas analyzer. By comparing the liquid membrane and film boiling method, determination of oxygenation results. Measured liquid membrane oxygenation is more effective than hollow fiber module method. Through the liquid membrane method, large quantities of hemoglobin can be treated in production, it also can reduce the loss of hemoglobin during purification, increase oxygen affinity.

Copyright
© 2015, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
Proceedings of the 4th International Conference on Mechatronics, Materials, Chemistry and Computer Engineering 2015
Series
Advances in Computer Science Research
Publication Date
December 2015
ISBN
978-94-6252-133-9
ISSN
2352-538X
DOI
https://doi.org/10.2991/icmmcce-15.2015.408How to use a DOI?
Copyright
© 2015, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

TY  - CONF
AU  - Chen Chen
AU  - Xiang Wang
AU  - Pei Wang
AU  - Donglai Qi
PY  - 2015/12
DA  - 2015/12
TI  - The study of carbon monoxide placenta blood hemoglobin oxygenation
BT  - Proceedings of the 4th International Conference on Mechatronics, Materials, Chemistry and Computer Engineering 2015
PB  - Atlantis Press
SN  - 2352-538X
UR  - https://doi.org/10.2991/icmmcce-15.2015.408
DO  - https://doi.org/10.2991/icmmcce-15.2015.408
ID  - Chen2015/12
ER  -