The Improvement of α-Amylase Enzyme Stability from Aspergillus fumigatus through Immobilization Method Using Hybrid Zeolite/Chitin Matrix

DOI

10.2991/978-94-6463-431-0_16How to use a DOI?

Keywords

Immobilization; α-amylase enzyme; Aspergillus fumigatus; hybrid zeolite/chitin matrix

Abstract

The stability of enzymes against pH and temperature is an important factor in the industry. Generally, enzymes can work on physiological conditions and unresistant to extreme pH and temperature conditions. To be used in industry, it is necessary to enhance enzyme stability. This research aims to enhance the stability of the α-amylase enzyme from Aspergillus fumigatus through immobilization using a hybrid matrix of zeolite and chitin. The α-amylase enzyme was isolated by centrifugation and purified using ammonium sulfate precipitation and dialysis. The purified α-amylase has an optimum temperature of 50℃ and an optimum pH of 6, while the immobilized α-amylase has an optimum temperature of 60℃ and an optimum pH of 5. The residual activity of the purified α-amylase after being incubated for 100 minutes at 50℃ was 17.08%, whereas the immobilized α-amylase after being incubated at 60℃ retained a residual activity of 67.30%. The purified α-amylase has a half-life (t½) of 37.067 minutes ± 0.000, while the immobilized α-amylase has a t½ of 173.287 minutes ± 0.000. The immobilized α-amylase from A. fumigatus using the hybrid zeolite/chitin matrix has better stability compared to the purified α-amylase; this is proved by a 4.68-fold increase in t½ compared to the purified enzyme. The immobilized enzyme still retained 47.54% residual activity after being used for six repeated cycles.

Copyright

© 2024 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Ella Gita Silviana
AU  - Yandri Yandri
AU  - Tati Suhartati
AU  - Bambang Irawan
AU  - Sutopo Hadi
PY  - 2024
DA  - 2024/05/30
TI  - The Improvement of α-Amylase Enzyme Stability from Aspergillus fumigatus through Immobilization Method Using Hybrid Zeolite/Chitin Matrix
BT  - Proceedings of selected papers of International Conference on Health, Science, and Environment (ICHSE 2023)
PB  - Atlantis Press
SP  - 147
EP  - 162
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-431-0_16
DO  - 10.2991/978-94-6463-431-0_16
ID  - Silviana2024
ER  -