Proceedings of selected papers of International Conference on Health, Science, and Environment (ICHSE 2023)

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16S rRNA Fragment of Metagenomic DNA, Antibacterial and Cellulase Activity from Garbage Enzymes of Sweet Orange (Citrus sinensis) Peels

Authors
I Nengah Wirajana1, 2, *, I Made Bramandita2, I Made Sukadana1, 2
1Chemistry Undergraduate Study Program, Faculty of Mathematics and Natural Sciences, Udayana University, Bali, Indonesia
2Chemistry Master Study Program, Faculty of Mathematics and Natural Sciences, Udayana University, Bali, Indonesia
*Corresponding author. Email: nengah_wirajana@unud.ac.id
Corresponding Author
I Nengah Wirajana
Available Online 30 May 2024.
DOI
10.2991/978-94-6463-431-0_17How to use a DOI?
Keywords
antibacterial; cellulase activity; metagenomic DNA; 16S rRNA
Abstract

Organic waste, such as fruit peels, has potential to be processed into useful products, for example through fermentation into garbage enzymes (GE) or also known as eco-enzymes. This study aims to determine 16S rRNA gene fragment amplicon of metagenomic DNA, antibacterial activity against the Staphylococcus aureus and Escherichia coli and cellulase activity from GE of sweet orange (Citrus sinensis) peels. Sweet orange peel was mixed with brown sugar and water in the ratio of 3:1:10 respectively, then fermented for 90 days (GE-90) and 105 days (GE-105). The metagenomic DNA isolate of GE was amplified by polymerase chain reaction (PCR) technique, antibacterial activity was tested using the agar diffusion well method, and cellulase activity was determined by the dinitrosalicylic acid (DNS) method. The results of GE-90 and GE-105 metagenomic DNA amplification using PCR was obtained 16S rRNA gene fragment of about 750 bp. Inhibition effectiveness of undiluted GE-90 against S. aureus and E. coli compared with amoxicillin 0.03% was 76.7 and 71.6%, respectively; while GE-105 was 68.7 and 56.6% respectively. Cellulase activity of GE-90 was 0.01186 ± 0.00077 U/mL; and 0.01384 ± 0.00113 U/mL for GE-105.

Copyright
© 2024 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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Volume Title
Proceedings of selected papers of International Conference on Health, Science, and Environment (ICHSE 2023)
Series
Advances in Biological Sciences Research
Publication Date
30 May 2024
ISBN
10.2991/978-94-6463-431-0_17
ISSN
2468-5747
DOI
10.2991/978-94-6463-431-0_17How to use a DOI?
Copyright
© 2024 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

Cite this article

risenwbib
TY  - CONF
AU  - I Nengah Wirajana
AU  - I Made Bramandita
AU  - I Made Sukadana
PY  - 2024
DA  - 2024/05/30
TI  - 16S rRNA Fragment of Metagenomic DNA, Antibacterial and Cellulase Activity from Garbage Enzymes of Sweet Orange (Citrus sinensis) Peels
BT  - Proceedings of selected papers of International Conference on Health, Science, and Environment (ICHSE 2023)
PB  - Atlantis Press
SP  - 163
EP  - 171
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-431-0_17
DO  - 10.2991/978-94-6463-431-0_17
ID  - Wirajana2024
ER  -