The Influence of Magnetic Fields Supply and Hygromycin Concentration to the Optimization of Folate Gene Putative Transformant Selection in Rice (cv. Fatmawati)
- DOI
- 10.2991/absr.k.210304.046How to use a DOI?
- Keywords
- Agrobacterium tumefaciens, Hygromycin, Magnetic Field Supply, Oryza sativa L, Transformation
- Abstract
The research was aimed to determine the influence of magnetic fields supply and hygromycin concentration on the optimization of folate gene putative transformant selection in rice (cv. Fatmawati). The Genetic transformation of rice used A. tumefaciens strain LBA4404 as a vector that contained plasmid pCambia1300-CGH1 construct through in planta transformation method using scutellum tissue explant. This research was conducted at the Laboratory of Plant Physiology and Biotechnology, Faculty of Agriculture, Sultan Ageng Tirtayasa University, Banten (February to April 2019). This research used a completely randomized design consisting of two factors. The first factor was the supply of a magnetic field, with two levels: without magnet and magnet. The second was the hygromycin concentrations with 4 levels: 25, 50, 75, and 100 mg.L-1. The results of the magnetic fields did not affect the transformation efficiency parameters and the percentage of explant deaths. The results of different hygromycin concentrations showed an effect on transformation efficiency parameters and percentage of explants deaths. The concentration of hygromycin 25 mg.L-1 had the highest average of transformation efficiency 100% based on the number of explants who passed the selection media suspected of being putative transformants and the low percentage of explants deaths 0%. In the lethal test of concentration-50 (LC50) hygromycin, the concentration of hygromycin 75 mg.L-1 caused up to 50% mortality in rice cv. Fatmawati explants. There had no interaction between the magnetic field supply and the hygromycin concentration on the parameters of transformation efficiency and the percentage of explant deaths.
- Copyright
- © 2021, the Authors. Published by Atlantis Press.
- Open Access
- This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
Cite this article
TY - CONF AU - Susiyanti AU - Winda Wati AU - Nurmayulis Nurmayulis AU - Sulastri Isminingsih AU - Sjaifuddin Sjaifuddin PY - 2021 DA - 2021/03/04 TI - The Influence of Magnetic Fields Supply and Hygromycin Concentration to the Optimization of Folate Gene Putative Transformant Selection in Rice (cv. Fatmawati) BT - Joint proceedings of the 2nd and the 3rd International Conference on Food Security Innovation (ICFSI 2018-2019) PB - Atlantis Press SP - 248 EP - 253 SN - 2468-5747 UR - https://doi.org/10.2991/absr.k.210304.046 DO - 10.2991/absr.k.210304.046 ID - 2021 ER -