A specific fluorescence probe for hypochlorous acid based on rhodamin101 thiosemicarbazides and its application for living cells imaging
Authors
Bo-Song Zhu, Zhi-Xiang Han, Tong-Liang Wu, Xiang-Yang Wu
Corresponding Author
Bo-Song Zhu
Available Online January 2016.
- DOI
- 10.2991/iccte-16.2016.195How to use a DOI?
- Keywords
- Rhodamine 101; Hypochlorous Acid; Bioimaging; Living cells
- Abstract
Utilizing the mechanism of HOCl-mediated cyclization of rhodamine 101 thiosemicarbazide (non-fluorescent) to rhodamine 101 oxadiazoles (fluorescent), we successfully developed a novel off-on far-red fluorescent probe (1) toward HOCl with high selectivity, great functionality at physiological pH and good cell-membrane permeability. Probe 1 was linearly proportional to the concentration of HOCl in the range of 1-10 M and the detection limit is 0.3 M. Moreover, the proposed probe (1) was successfully used for the detection of hypochlorous acid in HepG2 cells by fluorescent imaging.
- Copyright
- © 2016, the Authors. Published by Atlantis Press.
- Open Access
- This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
Cite this article
TY - CONF AU - Bo-Song Zhu AU - Zhi-Xiang Han AU - Tong-Liang Wu AU - Xiang-Yang Wu PY - 2016/01 DA - 2016/01 TI - A specific fluorescence probe for hypochlorous acid based on rhodamin101 thiosemicarbazides and its application for living cells imaging BT - Proceedings of the 2016 International Conference on Civil, Transportation and Environment PB - Atlantis Press SP - 1115 EP - 1118 SN - 2352-5401 UR - https://doi.org/10.2991/iccte-16.2016.195 DO - 10.2991/iccte-16.2016.195 ID - Zhu2016/01 ER -