The Immunostimulant Activities of the Gambir (Uncaria gambir Roxb) on Raw 264.7 Cell

Elsya Gustia; Yufri Aldi; Dira Hefni; Sefrianita Kamal; Dachriyanus; Fatma Sri Wahyuni

doi:10.2991/ahsr.k.211105.041

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The Immunostimulant Activities of the Gambir (Uncaria gambir Roxb) on Raw 264.7 Cell

Authors

Elsya Gustia¹, Yufri Aldi¹, Dira Hefni¹, Sefrianita Kamal², Dachriyanus¹, Fatma Sri Wahyuni¹^{, *}

¹Faculty of Pharmacy, Universitas Andalas, Kampus Limau Manis, 25163, Padang, West Sumatra, Indonesia

²Pharmacy Study Programme, Universitas Dharma Andalas Padang, West Sumatera, Indonesia.

^*Corresponding author. Email: fatmasriwahyuni@phar.unand.ac.id

Corresponding Author

Fatma Sri Wahyuni

Available Online 17 November 2021.

DOI: 10.2991/ahsr.k.211105.041 How to use a DOI?
Keywords: Uncaria gambir Roxb; RAW 264.7 cell; cell viability; immunostimulant
Abstract: Gambir (Uncaria gambir Roxb) is a plant that is known to have various activities, one of which is immunostimulant. This study aims to determine whether gambir from Payakumbuh affects the viability and immunostimulant activity on RAW 264.7 cells induced by lipopolysaccharide (LPS). This study was conducted in vitro with a concentration 1, 10, 100μg/mL on the viability and immunostimulatory activity of RAW 264.7 cells. Cell viability testing using the microtetrazolium (MTT) method aims to see whether the gambir sample used is safe and not toxic on RAW 264.7 cells by looking at the cell viability value >90%. Gambir 1,10, 100μg/mL was safe and non-toxic to RAW 264.7 cells with a viability value >90%. Thus, the concentration of gambir used can be further tested, namely the immunostimulant activity of gambir on RAW 264.7 cells induced by LPS looking at the levels of IL-6 (proinflammatory cytokines) compared with LPS as a control using the sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It was found that gambir 1,10, 100μg/mL could significantly increase the levels of IL-6 (p<0,05) which was tested with one-way ANOVA and continued with Duncan’s post hoc test. Based on these results, it can be concluded that gambir from Payakumbuh (Uncaria gambir Roxb) is safe and non-toxic and has immunostimulant activity by increasing the levels of IL-6 which were tested on RAW 264.7 cells induced by LPS.
Copyright: © 2021 The Authors. Published by Atlantis Press International B.V.
Open Access: This is an open access article under the CC BY-NC license.

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Volume Title: Proceedings of the 2nd International Conference on Contemporary Science and Clinical Pharmacy 2021 (ICCSCP 2021)
Series: Advances in Health Sciences Research
Publication Date: 17 November 2021
ISBN: 10.2991/ahsr.k.211105.041
ISSN: 2468-5739
DOI: 10.2991/ahsr.k.211105.041 How to use a DOI?
Open Access: This is an open access article under the CC BY-NC license.

Cite this article

ris enw bib

TY  - CONF
AU  - Elsya Gustia
AU  - Yufri Aldi
AU  - Dira Hefni
AU  - Sefrianita Kamal
AU  - Dachriyanus
AU  - Fatma Sri Wahyuni
PY  - 2021
DA  - 2021/11/17
TI  - The Immunostimulant Activities of the Gambir (Uncaria gambir Roxb) on Raw 264.7 Cell
BT  - Proceedings of the 2nd International Conference on Contemporary Science and Clinical Pharmacy 2021 (ICCSCP 2021)
PB  - Atlantis Press
SP  - 282
EP  - 288
SN  - 2468-5739
UR  - https://doi.org/10.2991/ahsr.k.211105.041
DO  - 10.2991/ahsr.k.211105.041
ID  - Gustia2021
ER  -

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