Chemical Contents Profile of Essential Oil from Calamansi (Citrus microcarpa Bunge) Peels and Leaves and Its Antibacterial Activities

DOI

10.2991/ahsr.k.211105.046How to use a DOI?

Keywords

essential oil; calamansi; GC-MS; antibacterial; terpenes compounds

Abstract

Citrus is a plant that has many health benefits and has been widely reported for its pharmacological effects. One of the citrus that has been reported to have antibacterial activity is calamansi (Citrus microcarpa Bunge). This study aims to determine the chemical content profile of the essential oil of fruit peels and leaves of calamansi. The essential oil was isolated by distillation of water and analyzed for chemical compound content by GC-MS. The methods used to determine the antibacterial activity of essential oils are diffusion and dilution methods. GC-MS results show that the main components of essential oil in the peels of the calamansi are D-limonene (29.52%), (R)-(+)-citronellal (13.76%), 3-isopropenyl-5,5-dimethyl-cyclopentene (8.88%), γ-terpinene (7.30%), citronellol (6.90%), and α-terpineol (4.61%) while the calamansi leaves consist of citronellal (25.74%), citronellol (12.94%), 3-carene (8.43), and β-phellandrene (4.89%). The essential oil of the fruit peels and leaves of calamansi showed antibacterial activity against MRSA, S. aureus, S. mutans, P. aeruginosa and E. coli bacteria. In the antibacterial activity test, the diffusion method showed that the essential oil of the fruit peels had moderate to strong inhibitory power, while the leaves of calamansi had very strong inhibitory power. In the dilution antibacterial activity test, it showed that the smallest MIC in the essential oil of fruit peels was a concentration of 0.39%, while the essential oil for leaves was 0.2%. The diffusion and dilution method of antibacterial activity test showed that the essential oil of the leaves was more potent than the peels of the calamansi.

Copyright

© 2021 The Authors. Published by Atlantis Press International B.V.

Open Access

This is an open access article under the CC BY-NC license.

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TY  - CONF
AU  - Elidahanum Husni
AU  - Fitri Yeni
AU  - Dachriyanus
PY  - 2021
DA  - 2021/11/17
TI  - Chemical Contents Profile of Essential Oil from Calamansi (Citrus microcarpa Bunge) Peels and Leaves and Its Antibacterial Activities
BT  - Proceedings of the 2nd International Conference on Contemporary Science and Clinical Pharmacy 2021 (ICCSCP 2021)
PB  - Atlantis Press
SP  - 314
EP  - 322
SN  - 2468-5739
UR  - https://doi.org/10.2991/ahsr.k.211105.046
DO  - 10.2991/ahsr.k.211105.046
ID  - Husni2021
ER  -