Proceedings of the 2nd International Conference on Contemporary Science and Clinical Pharmacy 2021 (ICCSCP 2021)

Antibacterial Activity of Ethyl Acetate Extracts from Mangrove Plants Rhizophora apiculata and Sonneratia alba — Associated Fungi

Authors
R Dyana Sartika, Annisa Rahma, Salsabillah Amelano, Dian Handayani*
Sumatran Biota Laboratory/Faculty of Pharmacy, Universitas Andalas, Kampus Limau Manis, Padang 25163, West Sumatera, Indonesia.
*Corresponding author. Email: dianhandayani@phar.unand.ac.id
Corresponding Author
Dian Handayani
Available Online 17 November 2021.
DOI
10.2991/ahsr.k.211105.049How to use a DOI?
Keywords
Mycobacterium tuberculosis; Aspergillus flavus; Aspergillus aculeatus; Penicillium rubens
Abstract

This study aims to explore the source of antibacterial compounds from mangrove endophytic fungi. Nine fungi were isolated from the leaves, barks, and roots of mangrove Rhizophora apiculata and Sonneratia alba from West Sumatera, Indonesia. The fungal isolate was cultivated in rice for 4-6 weeks, and subsequently, its secondary metabolites were extracted using ethyl acetate. The ethyl acetate extracts were tested against pathogenic bacteria; Bacillus cereus, Multi-Drug Resistant Pseudomonas aeuruginosa, Staphylococcus aureus, and Escherichia coli, and Mycobacterium tuberculosis. Seven extracts showed antibacterial activity at 5% (b/v %) concentration in the diffusion method. The highest inhibition was exhibited by the extract of RAA1 with the range of inhibition zone of 21.87 ± 0.81 mm – 26.12 ± 0.21 mm. Six fungal extracts (RAA2, RAA3, RAA4, RAK2, SaDa1, SaDa2) showed sensitivity to M. tuberculosis at concentrations of 3% and 5%. The potential fungi were identified molecularly by DNA amplification and sequencing of the fungal ITS region. The results of identification showed RAA1 as Aspergillus flavus, RAA4 as Aspergillus aculeatus, and SaDa1 as Penicillium rubens.

Copyright
© 2021 The Authors. Published by Atlantis Press International B.V.
Open Access
This is an open access article under the CC BY-NC license.

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Volume Title
Proceedings of the 2nd International Conference on Contemporary Science and Clinical Pharmacy 2021 (ICCSCP 2021)
Series
Advances in Health Sciences Research
Publication Date
17 November 2021
ISBN
978-94-6239-450-6
ISSN
2468-5739
DOI
10.2991/ahsr.k.211105.049How to use a DOI?
Copyright
© 2021 The Authors. Published by Atlantis Press International B.V.
Open Access
This is an open access article under the CC BY-NC license.

Cite this article

TY  - CONF
AU  - R Dyana Sartika
AU  - Annisa Rahma
AU  - Salsabillah Amelano
AU  - Dian Handayani
PY  - 2021
DA  - 2021/11/17
TI  - Antibacterial Activity of Ethyl Acetate Extracts from Mangrove Plants Rhizophora apiculata and Sonneratia alba — Associated Fungi
BT  - Proceedings of the 2nd International Conference on Contemporary Science and Clinical Pharmacy 2021 (ICCSCP 2021)
PB  - Atlantis Press
SP  - 336
EP  - 342
SN  - 2468-5739
UR  - https://doi.org/10.2991/ahsr.k.211105.049
DO  - 10.2991/ahsr.k.211105.049
ID  - DyanaSartika2021
ER  -