The Immunostimulant Effects of Alang-Alang (Imperata cylindrica) Roots Extract on BALB/c Male Mice (Mus musculus)

DOI

10.2991/absr.k.220406.068How to use a DOI?

Keywords

Humoral antibody; Immunostimulant; Imperata cylindrical; Leukocytes

Abstract

Alang-alang (Imperata cylindrica L.) is an annual rhizomatous grass and traditionally well known for its therapeutic values, especially in treating fever, muscle soreness, nosebleed and respiratory asphyxia. It has been reported to contain different classes of secondary metabolites and attracted scientists’ attention to assess its biological and pharmacological activities. Previous studies have shown that the roots extract of I. cylindrica possess antibacterial, anti-inflammatory, antihypertensive, anti-helminthic, antidiuretic, and antioxidant activities. While the objective of this research was to explore the immunostimulant potential of I. cylindrica roots extract by evaluating its effects on the BALB/c male mice immune system. A completely randomized design was used in this research with 5 treatments and 4 replications for each treatment. P0 was the negative or no-treatment control group; P1－P4 consisted of mice given I. cylindrica roots extract 0 mg/25 g BW (P1), 6.25 mg/25 g BW (P2), 12.5 mg/25 g BW (P3), and 18.75 mg/25 g BW (P4) daily for two-weeks and intraperitoneally injected with 1 % bovine red blood cell (BRBC) antigen suspension on day-15. The effects of the extract on total leukocyte count, differential leukocyte counts, and relative spleen weight measurement were studied in the mice, while activity on humoral antibody titer was evaluated in vitro. The results showed that all treated groups to which I. cylindrica was administrated had a greater count of leukocytes and P3 and P4 were demonstrated statistically significant differences (p<0.05) as compared to control groups. The effect of the extract in the number of lymphocytes was similar, whereas the number of monocyte and neutrophil of treatment groups was numerically increased but not statistically significant. It also substantially increased the humoral antibody titer value and relative spleen weight of the treatment groups. Overall, these investigations revealed the potential of the roots extract of I. cylindrica as an immunostimulant.

Copyright

© 2022 The Authors. Published by Atlantis Press International B.V.

Open Access

This is an open access article distributed under the CC BY-NC 4.0 license.

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TY  - CONF
AU  - Rosnizar Rosnizar
AU  - Fajar Muliani
AU  - Iskandar Muda Ramli
AU  - Kartini Eriani
PY  - 2022
DA  - 2022/05/02
TI  - The Immunostimulant Effects of Alang-Alang (Imperata cylindrica) Roots Extract on BALB/c Male Mice (Mus musculus)
BT  - Proceedings of the 7th International Conference on Biological Science (ICBS 2021)
PB  - Atlantis Press
SP  - 487
EP  - 493
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.220406.068
DO  - 10.2991/absr.k.220406.068
ID  - Rosnizar2022
ER  -