Proceedings of the 7th International Conference on Biological Science (ICBS 2021)

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Antibacterial Effectiveness of Synthesized Copper Nanoparticles by Ultrasonication Assisted Method

Authors
Ainul Fitria Mahmudah1, Yuni Kusumastuti2, Himawan Tri Bayu Murti Petrus2, Yekti Asih Purwestri3, 4, *
1Magister Program, Faculty of Biology, Universitas Gadjah Mada. Jl. Teknika Selatan, Sekip Utara, Sleman 55281, Yogyakarta, Indonesia
2Department of Chemical Engineering, Faculty of Engineering, Universitas Gadjah Mada Jl Grafika No. 2 Kampus UGM, 55281 Yogyakarta, Indonesia
3Faculty of Biology, Universitas Gadjah Mada. Jl. Teknika Selatan, Sekip Utara, Sleman 55281, Yogyakarta, Indonesia.
4Research Center for Biotechnology, Universitas Gadjah Mada. Jl. Teknika Utara, Barek, Sleman 55281, Yogyakarta, Indonesia.
*Corresponding author. E-mail: yekti@ugm.ac.id
Corresponding Author
Yekti Asih Purwestri
Available Online 2 May 2022.
DOI
10.2991/absr.k.220406.066How to use a DOI?
Keywords
Antibacterial; Copper Nanoparticles; Membrane integrity; Resistance
Abstract

Antibiotic resistance among pathogenic bacteria has become a problem in the medical community. Copper nanoparticles (CuNPs) have become one method of combating antibiotic resistance in bacteria. The antibacterial activity of Synthesized Copper nanoparticles (CuNPs) from sigma 774103-5G against various species of harmful bacteria, including Staphylococcus aureus FNCC 0047, Escherichia coli FNCC 0091, and Salmonella typhimurium FNCC 0134, was determined in this research. CuNPs were optimized by ultrasonication in order to enhance the release of Cu ions into the environment. CuNPs ranging in size from 60 to 80 nm were employed. CuNPs’ bactericidal impact can be assessed using a variety of methods, including the disk diffusion test, the Minimum Inhibitory Concentration (MIC), and the Minimum Bactericidal Concentration (MBC), and it can conducted with ultrasonification or non-ultrasonification respectively. The ultrasonication treatment was found to be efficient in boosting the antibacterial activity of all of the microorganisms examined. CuNPs demonstrated the best antibacterial activity against Staphylococcus aureus FNCC 0047, with a 23.7 mm inhibition zone diameter and a 37.5 g/mL MIC. The ultrasonication treatment was chosen for the preparation of CuNPs samples before further testing with the Alkaline Phosphatase Assay method, and the results showed that alkaline phosphatase enzyme as an indicator of cell damage was mostly detected in Staphylococcus aureus FNCC 0047 with a 4 hour exposure time. Membrane Integrity Staining with fluorescence microscopy viewing further revealed that cell damage increased as CuNPs exposure time increased.

Copyright
© 2022 The Authors. Published by Atlantis Press International B.V.
Open Access
This is an open access article distributed under the CC BY-NC 4.0 license.

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Volume Title
Proceedings of the 7th International Conference on Biological Science (ICBS 2021)
Series
Advances in Biological Sciences Research
Publication Date
2 May 2022
ISBN
10.2991/absr.k.220406.066
ISSN
2468-5747
DOI
10.2991/absr.k.220406.066How to use a DOI?
Copyright
© 2022 The Authors. Published by Atlantis Press International B.V.
Open Access
This is an open access article distributed under the CC BY-NC 4.0 license.

Cite this article

risenwbib
TY  - CONF
AU  - Ainul Fitria Mahmudah
AU  - Yuni Kusumastuti
AU  - Himawan Tri Bayu Murti Petrus
AU  - Yekti Asih Purwestri
PY  - 2022
DA  - 2022/05/02
TI  - Antibacterial Effectiveness of Synthesized Copper Nanoparticles by Ultrasonication Assisted Method
BT  - Proceedings of the 7th International Conference on Biological Science (ICBS 2021)
PB  - Atlantis Press
SP  - 471
EP  - 481
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.220406.066
DO  - 10.2991/absr.k.220406.066
ID  - Mahmudah2022
ER  -