Proceedings of the 7th International Conference on Biological Science (ICBS 2021)

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Comparison of the 12S rRNA and COI Regions of Mitochondrial DNA for eDNA Detection of Alligator Gar (Atractosteus spatula)

Authors
Patricia Agustin1, Andi Eko Maryanto1, 2, *, Noviar Andayani1, 2
1Department of Biology, Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Indonesia, Depok 16424, Indonesia
2Biodiversity and Environmental Genomics Research Cluster, Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Indonesia, Depok 16424, Indonesia
*Corresponding author. Email: andi.maryanto@ui.ac.id
Corresponding Author
Andi Eko Maryanto
Available Online 2 May 2022.
DOI
10.2991/absr.k.220406.062How to use a DOI?
Keywords
Atractosteus spatula; Detection; eDNA; Invasive; qPCR
Abstract

Alligator gar (Atractosteus spatula) has been introduced to many places outside its native range. The fish is considered as an invasive alien species that needs to be eradicated upon encounter in Indonesia due to its aggressive behavior. Despite existing regulations in Indonesia, the fish is still bred and widely distributed as a pet fish which increases the potential of being released into native waters. Non-invasive early detection of alligator gar is important as part of management efforts. This experiment was conducted to compare the 12S rRNA and COI regions of mitochondrial DNA for detection and estimation of alligator gar. Water samples (250 mL) were obtained from mesocosms containing one and three fish. The environmental DNA (eDNA) was filtered and extracted from the samples before amplified using quantitative PCR (qPCR). The eDNA concentrations amplified using 12S primers were higher than COI primers with 49.2×106–14.2×109 average copies/sample. There was negligible correlation between fish biomass and eDNA copy numbers amplified using 12S primers, but there was significant correlation (y = 330.6x − 1175, R2 = 0.3356, p < 0.05) between fish biomass and eDNA copy numbers amplified using COI primers. Thus, targeted qPCR using specific primers is more effective for detection and estimation of alligator gar in nature.

Copyright
© 2022 The Authors. Published by Atlantis Press International B.V.
Open Access
This is an open access article distributed under the CC BY-NC 4.0 license.

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Volume Title
Proceedings of the 7th International Conference on Biological Science (ICBS 2021)
Series
Advances in Biological Sciences Research
Publication Date
2 May 2022
ISBN
10.2991/absr.k.220406.062
ISSN
2468-5747
DOI
10.2991/absr.k.220406.062How to use a DOI?
Copyright
© 2022 The Authors. Published by Atlantis Press International B.V.
Open Access
This is an open access article distributed under the CC BY-NC 4.0 license.

Cite this article

risenwbib
TY  - CONF
AU  - Patricia Agustin
AU  - Andi Eko Maryanto
AU  - Noviar Andayani
PY  - 2022
DA  - 2022/05/02
TI  - Comparison of the 12S rRNA and COI Regions of Mitochondrial DNA for eDNA Detection of Alligator Gar (Atractosteus spatula)
BT  - Proceedings of the 7th International Conference on Biological Science (ICBS 2021)
PB  - Atlantis Press
SP  - 443
EP  - 448
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.220406.062
DO  - 10.2991/absr.k.220406.062
ID  - Agustin2022
ER  -