Highly sensitive fluorescent aptasensor for adenosine detection based on gold nanoparticle tagged molecular beacon
Authors
Liying Zheng, Songbai Zhang, Jinlin Lu, Xia Hu, Guangyu Shen, Xuewen Liu, Yuandao Chen, Liping Qiu, Shibiao Zhou
Corresponding Author
Liying Zheng
Available Online October 2015.
- DOI
- 10.2991/icadme-15.2015.57How to use a DOI?
- Keywords
- Molecular beacon, aptasensor, gold nanoparticle, fluorescent, adenosine
- Abstract
In this contribution, a sensitive fluorescent aptasensing strategy for the detection of adenosine is proposed based on structure switching of gold nanoparticle modified molecular beacon. Hairpin structured molecular beacon exhibits low background fluorescence since the fluorophore and nanoparticle quencher are in close proximity in the absence of adenosine. Binding with target molecules induces structure switching of molecular beacon, resulting in significant fluorescence increase due to separation of fluorophore and quencher. The fluorescence intensity is linear with adenosine concentration in a dynamic range from 90nM -1.5 M with a detection limit of 60 nM. Moreover, good reproducibility and high specificity are achieved.
- Copyright
- © 2015, the Authors. Published by Atlantis Press.
- Open Access
- This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
Cite this article
TY - CONF AU - Liying Zheng AU - Songbai Zhang AU - Jinlin Lu AU - Xia Hu AU - Guangyu Shen AU - Xuewen Liu AU - Yuandao Chen AU - Liping Qiu AU - Shibiao Zhou PY - 2015/10 DA - 2015/10 TI - Highly sensitive fluorescent aptasensor for adenosine detection based on gold nanoparticle tagged molecular beacon BT - Proceedings of the 5th International Conference on Advanced Design and Manufacturing Engineering PB - Atlantis Press SP - 287 EP - 290 SN - 2352-5401 UR - https://doi.org/10.2991/icadme-15.2015.57 DO - 10.2991/icadme-15.2015.57 ID - Zheng2015/10 ER -