Prepared polyclonal antibody of the semicarbazide
- 10.2991/ic3me-15.2015.90How to use a DOI?
- Nitrofurazone ;Semicarbazide; Hapten; Conjuguate; Polyclonal Antibody
Nitrofurazone residues in fishery products were dangerous for human health , semicarbazide (SEM) was the nitrofurazone metabolite could residues in fishery in a long time . To detect SEM residues in fishery products rapidly by immune method, preparation of high quality polyclonal antibody against SEM was the most critical step of project. We produced two kinds of antigens for SEM, using carrier protein bovine serum albumin (BSA) and ovalbumin (OVA) by a modified carbodiimide method, and then the hapten-protein conjugates were characterized by ultraviolet spectrophotometry in detecting qualitatively comparable hapten density, before being used for the immunization and detection purposes. The production of polyclonal antibodies (PcAbs) were sought following the generation of appropriate SEM-BSA conjugate. One PcAb against Semicarbazide (SEM) was produced and the titer polyclonal antibody could reach 105. In the optimized ELISA, the PcAb showed 50% inhibition at 25.0ng/mL for SEM in buffer.
- © 2015, the Authors. Published by Atlantis Press.
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- This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
Cite this article
TY - CONF AU - Xuan-Yun Huang AU - Dong-Mei Huang AU - You-Qiong Cai PY - 2015/08 DA - 2015/08 TI - Prepared polyclonal antibody of the semicarbazide BT - Proceedings of the 3rd International Conference on Material, Mechanical and Manufacturing Engineering PB - Atlantis Press SP - 458 EP - 461 SN - 2352-5401 UR - https://doi.org/10.2991/ic3me-15.2015.90 DO - 10.2991/ic3me-15.2015.90 ID - Huang2015/08 ER -