Determination of acrylamide in roasted coffee by UPLC-MS/MS
- https://doi.org/10.2991/ic3me-15.2015.73How to use a DOI?
- Ultra-high performance liquid chromatography-mass spectrometry; Acrylamide; Roasted coffee
A method for the determination of acrylamide in roasted coffee by UPLC-MS/MS was established. Acrylamide was extracted with water, and clean up on a HLB and MCX SPE cartridge, separated on a C18 column using acetonitrile-0.1% formic acid as mobile phase, and then detected under multiple reaction monitoring mode Deuterium labelled D3-acrylamide was used as internal standard. The recoveries ranged from 87.2 to 92.0 at three spiked concentrations with the relative standard deviations lower than 6 . The LOD and LOQ were 5 and 15 g/kg, respectively.
- © 2015, the Authors. Published by Atlantis Press.
- Open Access
- This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
Cite this article
TY - CONF AU - Shaodong Zeng AU - Tongyu Xu AU - Mingyue Wang AU - Chunliang Yang PY - 2015/08 DA - 2015/08 TI - Determination of acrylamide in roasted coffee by UPLC-MS/MS BT - Proceedings of the 3rd International Conference on Material, Mechanical and Manufacturing Engineering PB - Atlantis Press SP - 376 EP - 379 SN - 2352-5401 UR - https://doi.org/10.2991/ic3me-15.2015.73 DO - https://doi.org/10.2991/ic3me-15.2015.73 ID - Zeng2015/08 ER -