Detection of Pathogenic Bacteria in Shrimp Paste through an Enrichment Stage Using Nutrient Broth Medium

DOI

10.2991/978-94-6463-274-3_36How to use a DOI?

Keywords

detection; enrichment; pathogenic bacteria; shrimp paste

Abstract

Terasi products are one of the home industry products in Indonesia. Shrimp paste is produced from fermented small shrimp (called rebon) and small fish (called teri). As a food product marketed to the public, shrimp paste also has the potential to be contaminated with pathogens, both during production and after being sold. One of the pathogens that is highlighted in shrimp paste products comes from bacteria. In fact, the Indonesian Food and Drug Supervisory Agency (BPOM) emphasizes that specific pathogenic bacteria that you should be wary of can contaminate shrimp paste products. The sample is a packaged shrimp paste product that is ready to be marketed in Lombok Island. The enrichment stage was carried out using Nutrient Broth (NB) medium for 0 to 24 hours. The mixture was inoculated on Baird-Parker Agar (BPA) medium for detection of gram-positive pathogenic bacteria and Thiosulfate-Citrate-Bile Salts-Sucrose Agar (TCBS Agar) medium for detection of gram-negative pathogenic bacteria. The effect of the enrichment stage using NB medium resulted in a high increase in cell numbers. The highest increase occurred at 18 hours of enrichment, both in BPA and TCBS Agar medium. The enrichment method can be used as a preparation step for advanced applications such as molecular detection, cell activity assays, and culture collections.

Copyright

© 2023 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Firman Fajar Perdhana
AU  - Mutia Devi Ariyana
AU  - Lalu Unsunnidhal
AU  - Muhammad ‘Aidil Febriandito
AU  - Lalu Ahmad Wirayuda
AU  - Muhammad Aidil Fitriyan Fadjar Suryadi
AU  - Lulu Diani Zuhdia
PY  - 2023
DA  - 2023/10/27
TI  - Detection of Pathogenic Bacteria in Shrimp Paste through an Enrichment Stage Using Nutrient Broth Medium
BT  - Proceedings of the 7th International Conference on Food, Agriculture, and Natural Resources (IC-FANRES 2022)
PB  - Atlantis Press
SP  - 388
EP  - 394
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-274-3_36
DO  - 10.2991/978-94-6463-274-3_36
ID  - Perdhana2023
ER  -