Optimization of Culture Conditions to improve PHB Production of Methane-Utilizing Mixed Culture

DOI

10.2991/emim-16.2016.273How to use a DOI?

Keywords

Poly-?-hydroxybutyrate (PHB); Methane-utilizing mixed culture; Methanotrophic bacteria; High cell density

Abstract

Poly-?-hydroxybutyrate (PHB) can be produced by various species of bacteria as carbon and energy storage materials. This biopolyester has attracted increasing attentions as biodegradable plastics not only for its similar material properties to conventional plastics but also for its biodegradable properties. PHB has thermoplastic, can be used for food packaging film bag, packaging lining layer for drinks and fresh food tray, etc. Furthermore, PHB has lower oxygen and moisture permeability, which is benefit for food storage and transportation, so that it has a broad application prospect in the field of food packaging. In this peper, Methane-utilizing mixed culture with a high PHB storage capacity was gained from the biogas soil at DA Qing oil field as enriching source after domestication with the approach of the aerobic dynamic feeding (ADF?feast-famine condition). The community structure of Methane-utilizing mixed culture and the catalytic performance of the heterotrophs and methanotrophic bacteria isolated from the mixed culture were investigated. By adjusting the medium concentration of several important nutrients. Optimized medium suitable for cell growth in the first stage and second stage of cellular accumulation of PHB were obtained. By optimizing the supply of carbon sources and the composition of the culture medium, high cell density and yield of PHB can be obtained.

Copyright

© 2016, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Wei Zhang
AU  - Qiannan Wang
AU  - Jiaying Xin
PY  - 2016/04
DA  - 2016/04
TI  - Optimization of Culture Conditions to improve PHB Production of Methane-Utilizing Mixed Culture
BT  - Proceedings of the 6th International Conference on Electronic, Mechanical, Information and Management Society
PB  - Atlantis Press
SP  - 1340
EP  - 1344
SN  - 2352-538X
UR  - https://doi.org/10.2991/emim-16.2016.273
DO  - 10.2991/emim-16.2016.273
ID  - Zhang2016/04
ER  -