Expression of Beta-Adrenergic Receptor in Glioma LN229 Cells and Its Effect on Cell Proliferation

DOI

10.2991/emcm-16.2017.140How to use a DOI?

Keywords

Glioma; Adrenergic receptor; Proliferation; Norepinephrine; Stress hormone

Abstract

Objective To investigate the expression of beta-adrenergic receptor( -AR) in glioma cells and the effect of blocking -adrenergic receptors on glioma cell proliferation. Methods: The expression of beta-adrenergic receptor in glioma LN229 and U251 cells were detected by real time PCR and Western Blotting. The proliferation of glioma cells was analyzed by MTT assay after they were exposed to norepinephrine and propranolol(non-selective blocker of beta adrenergic receptors) alone, or in combination, respectively. Results: The LN229 and U251 cells both expressed beta 1 and beta 2 adrenergic receptors, but beta2-receptor showed a relatively strong expression while beta1-receptor showed a relatively weak expression in LN229cells. The proliferation of glioma cells was significantly inhibited by propranolol which acts as a nonspecific blocker of beta-adrenergic receptors, with a certain time and concentration-dependent matter toward higher inhibition, and the differences had statistically significance (all P <0.05). The proliferation LN299 cells were significantly increased after norepinephrine, which was in a concentration-dependent manner antagonized by propranolol co treatment (P <0.05). Conclusion: Both adrenergic beta1 and beta2 are expression of glioma cells, and non-selective blockage of beta adrenergic receptor can effectively inhibit the proliferation of glioma cells.

Copyright

© 2017, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Junhong Dong
AU  - Ping Wang
AU  - Qingluan Xu
AU  - Kunpeng Lv
AU  - Yu Wang
PY  - 2017/02
DA  - 2017/02
TI  - Expression of Beta-Adrenergic Receptor in Glioma LN229 Cells and Its Effect on Cell Proliferation
BT  - Proceedings of the 2016 7th International Conference on Education, Management, Computer and Medicine (EMCM 2016)
PB  - Atlantis Press
SN  - 2352-538X
UR  - https://doi.org/10.2991/emcm-16.2017.140
DO  - 10.2991/emcm-16.2017.140
ID  - Dong2017/02
ER  -