Combined Application of SRS and Photoacoustic Imaging in Vivo Leaf Tissue in Situ Detection System

DOI

10.2991/eee-19.2019.43How to use a DOI?

Keywords

Photoacoustic imaging, Raman spectroscopy, In situ detection, Living tissue

Abstract

Absorption and scattering are two common methods to characterize the physical and chemical properties of substances. However, the combined application of the two techniques in situ detection is difficult. Taking biological tissues as an example, proteins and pigments absorb visible light strongly, and the scattering characteristics of soft tissues are obvious. Absorption and scattering can be used to characterize the physical and chemical properties of substances. Scattering characteristics of tissues can reveal information such as cell structure, and the absorption characteristics of tissues can often reflect information such as cell metabolism. The combination of light scattering and photoacoustic effect can extract all kinds of physical and chemical information of tissue and molecule at the same time and detect them in situ. Due to the influence of the detected objects, the combined application of the two in situ detection techniques is difficult. Therefore, in order to compensate for each other's shortcomings, the photoacoustic effect caused by optical absorption and Raman scattering are used to detect living tissues, and a combined experimental system of Raman and photoacoustic imaging in situ detection is proposed. Raman and photoacoustic signals are synchronously stimulated by high repetition frequency pulsed laser and multiplexed by optical system. On the basis of experiments of adjusting repetition frequency and power of pulsed laser, the effects of parameters of pulsed laser on Raman and photoacoustic signals are analyzed. The results are of great significance for analyzing optical and acoustic signals of living tissues.

Copyright

© 2019, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Hong-peng Wang
AU  - Ru-jun Yuan
AU  - Xiong Wan
PY  - 2019/07
DA  - 2019/07
TI  - Combined Application of SRS and Photoacoustic Imaging in Vivo Leaf Tissue in Situ Detection System
BT  - Proceedings of the 2nd International Conference on Electrical and Electronic Engineering (EEE 2019)
PB  - Atlantis Press
SP  - 268
EP  - 273
SN  - 2352-5401
UR  - https://doi.org/10.2991/eee-19.2019.43
DO  - 10.2991/eee-19.2019.43
ID  - Wang2019/07
ER  -