T(-786)C Polymorphism of NOS3 Gene in the Yakut Population
- DOI
- 10.2991/ahsr.k.220103.042How to use a DOI?
- Keywords
- T(-786)C Polymorphism; Endothelial NOS; NOS3 gene; Yakut population
- Abstract
Endothelial NOS (nitric oxide synthase 3) is an enzyme that synthesizes nitric oxide, which in turn plays a crucial role in regulating vascular tone, cell proliferation, leukocyte adhesion, and platelet aggregation. This enzyme in humans is encoded by NOS3 gene. The aim of our research was to study T (-786) C polymorphism of NOS3 gene in healthy individuals of the Yakut population. The experimental part of the research was carried out in “Molecular Medicine and Human Genetics” of the NEFU Medical Institute. In total, 270 DNA samples of healthy Yakuts were examined (71 men: women 199, average age 49.18 ± 14.36 years). The single nucleotide polymorphism was determined by real-time PCR with fusion of fluorescent labeled hybridization products followed by analysis of melting curves. As a result of genotyping T (-786) C polymorphism of NOS3 gene, the predominance of T allele (95.37 %) was established among the Yakut population. Among all examined patients, carriers of the homozygous TT genotype were dominated – 246 people (91.11 %). The heterozygous TC genotype was 8.52 % (23), whereas the homozygous C allele genotype occurred only in 0.37 % of cases (1). When compared with populations of the world, according to the “1000 genomes,” C allele among the Yakuts was less common than among the population of North America, South America, Europe, India, Oceania, Africa and Asia. Thus, the Yakut population is characterized by a unique distribution of genotypes of T (-786) C polymorphism of NOS3 gene among populations of the world.
- Copyright
- © 2022 The Authors. Published by Atlantis Press International B.V.
- Open Access
- This is an open access article under the CC BY-NC license.
Cite this article
TY - CONF AU - Julia Solovieva AU - Natalia Borisova PY - 2022 DA - 2022/01/17 TI - T(-786)C Polymorphism of NOS3 Gene in the Yakut Population BT - Proceedings of the Conference on Health and Wellbeing in Modern Society (CHW 2021) PB - Atlantis Press SP - 209 EP - 213 SN - 2468-5739 UR - https://doi.org/10.2991/ahsr.k.220103.042 DO - 10.2991/ahsr.k.220103.042 ID - Solovieva2022 ER -