Analysis on the Performance of the Balb/c 3T3 Two-stage Assay in a Miniature Culture Area and in a Perfusion Culture Model

DOI

10.2991/bst-16.2016.17How to use a DOI?

Keywords

CTAs, Balb/c 3T3, Perfusion cell culture.

Abstract

The Balb/c 3T3 cell transformation assay (CTA) is presently undergoing extensive pre-validation testing but its potential for routine chemical screening remains to be fully exploited due it being more costly and more time consuming than other CTAs. To downscale the assay 24-well plates were used to score foci and then compared to the standard T25 culture bottles. Furthermore, it was also attempted an adaptation to a perfusion cell culture model in order to achieve a closer equivalent to the in vivo carcinogenic processes. The miniaturized approach was successfully correlated to the standard technique. A reproduction in smaller culture plates can provide an alternative for a faster and more practicable handling by smaller laboratories. The assay failed to produce positive results in the fluidic model and further testing is necessary to determine the role medium flow in the in vitro carcinogenesis process.

Copyright

© 2016, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Vania H. M. Teofilo
AU  - Jin-Tao Li
AU  - Xin-Hui Liu
AU  - Ru-Gang Zhong
AU  - Si Ye
AU  - Zheng-Bo Zhao
AU  - Yi Zeng
PY  - 2016/01
DA  - 2016/01
TI  - Analysis on the Performance of the Balb/c 3T3 Two-stage Assay in a Miniature Culture Area and in a Perfusion Culture Model
BT  - Proceedings of the 2016 International Conference on Biological Sciences and Technology
PB  - Atlantis Press
SP  - 104
EP  - 112
SN  - 2468-5747
UR  - https://doi.org/10.2991/bst-16.2016.17
DO  - 10.2991/bst-16.2016.17
ID  - Teofilo2016/01
ER  -