The Appropriate Dose of Curcumin Induces The Cell Differentiation of Cortical Neural Stem Cells (NSCs)

DOI

10.2991/978-94-6463-525-6_22How to use a DOI?

Keywords

Gene expression; MAP gene; Nestin gene; qPCR; SOX-2 gene

Abstract

Research on herbal curcumin is still developing, one of which is its role in stimulating the proliferation and differentiation of neural stem cells (NSC). The study used curcumin to stimulate neural stem cell proliferation to obtain the optimal dose for the proliferation process. We used the concentration of curcumin in this study was 0.1 µM, 0.5 µM, one µM, and two µM. The positive control used Synthetic growth factors are Basic Fibroblast Growth Factor (bFGF), Epidermal Growth Factor (EGF), and heparin. Meanwhile, Dimethyl Sulfoxide (DMSO) solution served as a negative control. Analysis of NSC proliferation ability using the water-soluble tetrazolium salt test (WST-1). As a marker for neural stem cell proliferation, the expression of the microtubule associated protein 2 (MAP-2), Nestin gene, and the sex-determining Y-box 2 (SOX2) gene by RT-PCR. Cell morphology analysis showed that cells proliferated optimally at a dose of 0.5 μM curcumin. One-way ANOVA analysis and the results of the Tukey post hoc test on the WST-1 test showed that a curcumin concentration of 0.5 μM was optimal to stimulate cell proliferation. The highest Sox2, MAP-2, and Nestin gene expression test results occurred with 0.5 μM curcumin. The results showed that a concentration of 0.5 μM of curcumin could stimulate NSC cell proliferation as well as stimulation by synthetic growth factors.

Copyright

© 2024 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Titta Novianti
AU  - Muhamad Panji Januarsyah Kustiawan
AU  - Ita Margaretha
PY  - 2024
DA  - 2024/10/29
TI  - The Appropriate Dose of Curcumin Induces The Cell Differentiation of Cortical Neural Stem Cells (NSCs)
BT  - Proceedings of the 2023 Brawijaya International Conference (BIC 2023)
PB  - Atlantis Press
SP  - 195
EP  - 200
SN  - 2352-5428
UR  - https://doi.org/10.2991/978-94-6463-525-6_22
DO  - 10.2991/978-94-6463-525-6_22
ID  - Novianti2024
ER  -