Proceedings of the 2016 International Conference on Biomedical and Biological Engineering

Secondary Metabolism Variation of a Marine Fungus Following Treatment with Dielectric Barrier Discharge Plasma and Chemical Mutagens

Authors
Yi Zhang, Lixue Wen, Haiyan Bao, Yingying Nie, Yan Feng, Zhilong Xiu
Corresponding Author
Yi Zhang
Available Online July 2016.
DOI
https://doi.org/10.2991/bbe-16.2016.40How to use a DOI?
Keywords
Marine fungus, Dielectric barrier discharge plasma, Chemical mutagens, Secondary metabolic fingerprints.
Abstract
To activate cryptic secondary metabolic genes is a crucial issue in the search for new natural products from microorganisms. For this purpose, in the present study, a marine fungal strain Penicillium chrysogenum WP-1 was treated with dielectric barrier discharge plasma, chemical mutagens, and their combination. Seven mutants with higher antibacterial activities were chosen among the colonies that survived the different treatments. As revealed by high resolution LC-MS analysis, their secondary metabolic fingerprints displayed significant variations in the numbers of total distinguishable peaks (metabolites), newly generated metabolites, nitrogenated compounds, compounds with skeletons larger than C20, compounds with molecular weights greater than 400 Da, and in the yields of the original metabolites. Additionally, different mutagenesis treatments resulted in different metabolite profiles. Moreover, most of these mutants showed morphological variations compared with the original strain. This study reveals that the plasma and plasma-chemical mutagen combination are effective methodsto activate silent fungalsecondary metabolites. It lays the foundation for the application of plasma mutagenesis in marine fungal natural product research.
Open Access
This is an open access article distributed under the CC BY-NC license.

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Proceedings
International Conference on Biomedical and Biological Engineering
Part of series
Advances in Biological Sciences Research
Publication Date
July 2016
ISBN
978-94-6252-216-9
ISSN
2468-5747
DOI
https://doi.org/10.2991/bbe-16.2016.40How to use a DOI?
Open Access
This is an open access article distributed under the CC BY-NC license.

Cite this article

TY  - CONF
AU  - Yi Zhang
AU  - Lixue Wen
AU  - Haiyan Bao
AU  - Yingying Nie
AU  - Yan Feng
AU  - Zhilong Xiu
PY  - 2016/07
DA  - 2016/07
TI  - Secondary Metabolism Variation of a Marine Fungus Following Treatment with Dielectric Barrier Discharge Plasma and Chemical Mutagens
BT  - International Conference on Biomedical and Biological Engineering
PB  - Atlantis Press
SN  - 2468-5747
UR  - https://doi.org/10.2991/bbe-16.2016.40
DO  - https://doi.org/10.2991/bbe-16.2016.40
ID  - Zhang2016/07
ER  -