In Vivo Study, Effect Combination of Lactobacillus spp and Doxycycline to Eliminate of Vibrio cholerae

DOI

10.2991/978-94-6463-060-2_6How to use a DOI?

Keywords

In vivo study; Lactobacillus spp; Vibrio cholerae

Abstract

Infection by Vibrio cholerae is a major public health problem confronting developing countries. One of possible solution for the problems could come from probiotic which have been reported to reduce diarrhea. Aim of the research to determine effect combination of Lactobacillus spp and Doxycycline to eliminate of Vibrio cholerae in vivo. This research was an experimental study. Twenty five male Swiss Webster mouse were divided into five groups: K1 (negative control), K2 (positive control), K3 (treated by Doxycycline), and K4 (treated by Lactobacillus spp) and K5 (treated by combination of Lactobacillus spp and Doxycycline). Mouses are infected by Vibrio cholera until symptom arise and then treated for 3 days. Interval between treated Lactobacillus spp and Doxycycline was an hour. Feces was cultured on TCBS (Thiosulfate-citrate-bile salts-sucrose) media and then the colonies were counted by Total Plate Count method (TPC). Test of One-Way Anova (α < 0,05) and post-hoc test (α = 0,05) was applied. LSD post hoc test showed there were significantly difference between the group K1, K2 and K4, but K3 dan K5 were not significant. Group which treated by combination of Lactobacillus spp and Doxycycline reduced number of Vibrio cholerae lowest than another groups but not significant than treated by Doxycycline only. Lactobacillus spp. Could eliminate Vibrio cholerae because it changed intraluminal acidity, produced antimicrobial substance, competition to absorb nutrition and attachment at the intestinal enterocyte receptor. Need longer interval between treated Lactobacillus spp and Doxycycline. One hour interval not enough for Lactobacillus spp to work in gastrointestinal tract.

Copyright

© 2023 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Eka Noneng Nawangsih
AU  - Dinyar Supiadi Wijaya
AU  - Siska Telly Pratiwi
AU  - Rachmayanti Nur
PY  - 2022
DA  - 2022/12/16
TI  - In Vivo Study, Effect Combination of Lactobacillus spp and Doxycycline to Eliminate of Vibrio cholerae
BT  - Proceedings of The 13th Annual Scientific Conference of Medical Faculty, Universitas Jenderal Achmad Yani (ASCMF 2022)
PB  - Atlantis Press
SP  - 31
EP  - 36
SN  - 2468-5739
UR  - https://doi.org/10.2991/978-94-6463-060-2_6
DO  - 10.2991/978-94-6463-060-2_6
ID  - Nawangsih2022
ER  -