Local Material-Based DNA Experiment (LMBE)

Viable Alternative Approach for Biochemistry Laboratory

DOI

10.2991/assehr.k.210715.065How to use a DOI?

Keywords

biochemistry laboratory, creative thinking, DNA, electrophoresis, hands-on learning, local material-based experiment

Abstract

The COVID-19 pandemic has led to the implementation of e-learning to ensure the teaching process does not halt utterly due to the crisis. However, e-learning courses alone are less adequate to provide hands-on skills regarding laboratory experiments. The course designed emphasizes the development of student practical chemistry skills and creativity in conducting isolation and detection of DNA at home. The context of “varieties of tomatoes” provides students with an opportunity to study how genetics affects phenotype and how to see fruits DNA through an experiment. In this experiment, students were exposed to scientific methods and started their activity by defining the problem. The lab format also provides students to analyze the basic principle functions of each reagent used for DNA extraction and was challenged to explore alternative compounds to substitute standard chemicals. Afterwards, the students designed local material-based experiments (LMBE), as the way to develop creative and practical chemistry skills including critical analysis on the alternative experimental design, primary results interpretation and analysis, and scientific writing. In the second part, the students were introduced to learn the principles of electrophoresis as one of the molecular genetics techniques and built their homemade electrophoresis to visualize the extracted DNA from previous activity. To this end, the LMBE is serving an alternative approach to the aim of developing students’ practical and creativity skills in biochemistry laboratory during stay at home.

Copyright

© 2021, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Florentina Maria Titin Supriyanti
AU  - Gun Gun Gumilar
AU  - Heli Siti Halimatul Munawaroh
AU  - Rahmat Setiadi
PY  - 2021
DA  - 2021/07/17
TI  - Local Material-Based DNA Experiment (LMBE)
BT  - Proceedings of the 5th Asian Education Symposium 2020 (AES 2020)
PB  - Atlantis Press
SP  - 308
EP  - 312
SN  - 2352-5398
UR  - https://doi.org/10.2991/assehr.k.210715.065
DO  - 10.2991/assehr.k.210715.065
ID  - Supriyanti2021
ER  -