Nanosphere's Verigene® Blood Culture Assay to Detect Multidrug-Resistant Gram-Negative Bacterial Outbreak: A Prospective Study on 79 Hematological Patients in a Country with High Prevalence of Antimicrobial Resistance

Raffaella Greco; Maria Chiara Barbanti; Nicasio Mancini; Laura Infurnari; Renée Pasciuta; Alessandra Forcina; Chiara Oltolini; Gabriele Casirati; Daniele Mannina; Fabio Giglio; Carlo Messina; Mara Morelli; Francesca Lorentino; Sara Mastaglio; Tommaso Perini; Luca Vago; Paolo Scarpellini; Matteo Giovanni Carrabba; Maria Teresa Lupo Stanghellini; Sarah Marktel; Andrea Assanelli; Massimo Bernardi; Consuelo Corti; Jacopo Peccatori; Massimo Clementi; Fabio Ciceri

doi:10.2991/chi.d.190321.001

<Previous Article In Issue

Next Article In Issue>

Volume 1, Issue 2, June 2019, Pages 120 - 123

Nanosphere's Verigene^® Blood Culture Assay to Detect Multidrug-Resistant Gram-Negative Bacterial Outbreak: A Prospective Study on 79 Hematological Patients in a Country with High Prevalence of Antimicrobial Resistance

Authors

Raffaella Greco¹^{, †}, Maria Chiara Barbanti¹^{, †}, Nicasio Mancini², Laura Infurnari², Renée Pasciuta², Alessandra Forcina¹, Chiara Oltolini³, Gabriele Casirati¹, Daniele Mannina¹, Fabio Giglio¹, Carlo Messina¹, Mara Morelli¹, Francesca Lorentino¹, Sara Mastaglio¹, Tommaso Perini¹, Luca Vago¹, Paolo Scarpellini³, Matteo Giovanni Carrabba¹, Maria Teresa Lupo Stanghellini¹, Sarah Marktel¹, Andrea Assanelli¹, Massimo Bernardi¹, Consuelo Corti¹, Jacopo Peccatori¹, Massimo Clementi²^{, 4}^{, †}, Fabio Ciceri¹^{, 4}^{, *}^{, †}

¹Unit of Hematology and Bone Marrow Transplantation, IRCCS San Raffaele Scientific Institute, Milano, Italy

²Laboratory of Microbiology and Virology, IRCCS San Raffaele Scientific Institute, Milano, Italy

³Infectious Disease Unit, IRCCS San Raffaele Scientific Institute, Milano, Italy

⁴Vita-Salute San Raffaele University, Milano, Italy

^†

Equal contribution

^*Corresponding author. Email: ciceri.fabio@hsr.it

Corresponding Author

Fabio Ciceri

Received 20 February 2019, Accepted 18 March 2019, Available Online 11 June 2019.

DOI: 10.2991/chi.d.190321.001 How to use a DOI?
Keywords: Sepsis; Hematological patients; Molecular diagnosis; Carbapenem resistance
Abstract: Infections are a major cause of morbidity and mortality in hematological patients. We prospectively tested a new molecular assay (Verigene^®) in 79 consecutive hematological patients, with sepsis by gram-negative bacteria. A total of 82 gram-negative microorganisms were isolated by blood cultures, of which 76 cases were mono-microbial. Considering the bacteria detectable by the system, the concordance with standard blood cultures was 100%. Resistance genes were detected in 20 of the isolates and 100% were concordant with the phenotypic antibiotic resistance. Overall, this new assay correctly identified 66/82 of all the gram-negative pathogens, yielding a general sensitivity of 80.5%, and providing information on genetic antibiotic resistance in a few hours. This new molecular assay could ameliorate patient management, resulting in a more rational use of antibiotics.
Open Access: This is an open access article distributed under the CC BY-NC 4.0 license (http://creativecommons.org/licenses/by-nc/4.0/).

Download article (PDF)
View full text (HTML)

<Previous Article In Issue

Next Article In Issue>

Journal: Clinical Hematology International
Volume-Issue: 1 - 2
Pages: 120 - 123
Publication Date: 2019/06/11
ISSN (Online): 2590-0048
DOI: 10.2991/chi.d.190321.001 How to use a DOI?
Open Access: This is an open access article distributed under the CC BY-NC 4.0 license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

ris enw bib

TY  - JOUR
AU  - Raffaella Greco
AU  - Maria Chiara Barbanti
AU  - Nicasio Mancini
AU  - Laura Infurnari
AU  - Renée Pasciuta
AU  - Alessandra Forcina
AU  - Chiara Oltolini
AU  - Gabriele Casirati
AU  - Daniele Mannina
AU  - Fabio Giglio
AU  - Carlo Messina
AU  - Mara Morelli
AU  - Francesca Lorentino
AU  - Sara Mastaglio
AU  - Tommaso Perini
AU  - Luca Vago
AU  - Paolo Scarpellini
AU  - Matteo Giovanni Carrabba
AU  - Maria Teresa Lupo Stanghellini
AU  - Sarah Marktel
AU  - Andrea Assanelli
AU  - Massimo Bernardi
AU  - Consuelo Corti
AU  - Jacopo Peccatori
AU  - Massimo Clementi
AU  - Fabio Ciceri
PY  - 2019
DA  - 2019/06/11
TI  - Nanosphere's Verigene® Blood Culture Assay to Detect Multidrug-Resistant Gram-Negative Bacterial Outbreak: A Prospective Study on 79 Hematological Patients in a Country with High Prevalence of Antimicrobial Resistance
JO  - Clinical Hematology International
SP  - 120
EP  - 123
VL  - 1
IS  - 2
SN  - 2590-0048
UR  - https://doi.org/10.2991/chi.d.190321.001
DO  - 10.2991/chi.d.190321.001
ID  - Greco2019
ER  -

download .riscopy to clipboard