Nanosphere's Verigene® Blood Culture Assay to Detect Multidrug-Resistant Gram-Negative Bacterial Outbreak: A Prospective Study on 79 Hematological Patients in a Country with High Prevalence of Antimicrobial Resistance
- 10.2991/chi.d.190321.001How to use a DOI?
- Sepsis; Hematological patients; Molecular diagnosis; Carbapenem resistance
Infections are a major cause of morbidity and mortality in hematological patients. We prospectively tested a new molecular assay (Verigene®) in 79 consecutive hematological patients, with sepsis by gram-negative bacteria. A total of 82 gram-negative microorganisms were isolated by blood cultures, of which 76 cases were mono-microbial. Considering the bacteria detectable by the system, the concordance with standard blood cultures was 100%. Resistance genes were detected in 20 of the isolates and 100% were concordant with the phenotypic antibiotic resistance. Overall, this new assay correctly identified 66/82 of all the gram-negative pathogens, yielding a general sensitivity of 80.5%, and providing information on genetic antibiotic resistance in a few hours. This new molecular assay could ameliorate patient management, resulting in a more rational use of antibiotics.
- © 2019 International Academy for Clinical Hematology. Publishing services by Atlantis Press International B.V.
- Open Access
- This is an open access article distributed under the CC BY-NC 4.0 license (http://creativecommons.org/licenses/by-nc/4.0/).
Download article (PDF)
View full text (HTML)
Cite this article
TY - JOUR AU - Raffaella Greco AU - Maria Chiara Barbanti AU - Nicasio Mancini AU - Laura Infurnari AU - Renée Pasciuta AU - Alessandra Forcina AU - Chiara Oltolini AU - Gabriele Casirati AU - Daniele Mannina AU - Fabio Giglio AU - Carlo Messina AU - Mara Morelli AU - Francesca Lorentino AU - Sara Mastaglio AU - Tommaso Perini AU - Luca Vago AU - Paolo Scarpellini AU - Matteo Giovanni Carrabba AU - Maria Teresa Lupo Stanghellini AU - Sarah Marktel AU - Andrea Assanelli AU - Massimo Bernardi AU - Consuelo Corti AU - Jacopo Peccatori AU - Massimo Clementi AU - Fabio Ciceri PY - 2019 DA - 2019/06/11 TI - Nanosphere's Verigene® Blood Culture Assay to Detect Multidrug-Resistant Gram-Negative Bacterial Outbreak: A Prospective Study on 79 Hematological Patients in a Country with High Prevalence of Antimicrobial Resistance JO - Clinical Hematology International SP - 120 EP - 123 VL - 1 IS - 2 SN - 2590-0048 UR - https://doi.org/10.2991/chi.d.190321.001 DO - 10.2991/chi.d.190321.001 ID - Greco2019 ER -