Artery Research

Volume 12, Issue C, December 2015, Pages 6 - 6

P1.15 CONDITIONAL INACTIVATION OF INTEGRIN AV SUBUNIT IN VASCULAR SMOOTH MUSCLE CELLS REGULATES FIBROSIS IN VESSELS

Authors
Ekaterina Belozertseva*1, Melusine Didelot1, Amel Mohamadi1, Zhenlin Li2, Huguette Louis1, Jean-Baptiste Michel3, Véronique Regnault1, Patrick Lacolley1
1UMR_S 1116, Vandoeuvre-les-Nancy, France
2UMR 8256, Paris, France
3UMR_S 1148, Paris, France
Available Online 23 November 2015.
DOI
https://doi.org/10.1016/j.artres.2015.10.207How to use a DOI?
Abstract

Integrin avb3 is expressed at high density in vascular smooth muscle cells (VSMCs). It functions as a receptor for adhesion proteins in VSMCs which phenotypic modulation plays a pivotal role in arteriosclerosis and atherosclerosis.

The aim was to study the role of integrin avb3 in angiotensin II (Ang II)-induced arterial fibrosis in mice and in human samples of atherosclerotic arteries in situ. Transgenic mice conditionally inactivated for integrin av subunit in VSMCs (avSMKO) were treated with Ang II (1,5 mg/kg/day) for 4 weeks. Immunostained slices of atherosclerotic plaques at different stages of development and primary cultures of human aortic VSMCs were used.

At baseline, blood pressure was lower in avSMKO compared to control (WT) mice. Isobaric carotid distensibility was increased and remained higher in avSMKO in response to Ang II. The increase in collagen content in response to Ang II was lower in avSMKO than in WT (15 vs 36%) for similar increase in blood pressure (20 mmHg) and arterial wall hypertrophy.

The immunohistochemistry of aortic slices showed stronger staining for integrin avb3 in atherosclerotic plaques compared to healthy aortas. In VSMC cultures, the mRNA of av was decreased.

In conclusion, these results show that avb3 is strongly expressed in neointimal proliferation and in fibrous plaques. The av integrin subunit seems to regulate arterial fibrosis in response to hypertension and plaque growth. Low RNA quantities of av subunit of VSMCs contrasted with strong protein staining in plaques suggesting the participation of inflammatory cells in the synthesis of this integrin.

Open Access
This is an open access article distributed under the CC BY-NC license.

Journal
Artery Research
Volume-Issue
12 - C
Pages
6 - 6
Publication Date
2015/11
ISSN (Online)
1876-4401
ISSN (Print)
1872-9312
DOI
https://doi.org/10.1016/j.artres.2015.10.207How to use a DOI?
Open Access
This is an open access article distributed under the CC BY-NC license.

Cite this article

TY  - JOUR
AU  - Ekaterina Belozertseva*
AU  - Melusine Didelot
AU  - Amel Mohamadi
AU  - Zhenlin Li
AU  - Huguette Louis
AU  - Jean-Baptiste Michel
AU  - Véronique Regnault
AU  - Patrick Lacolley
PY  - 2015
DA  - 2015/11
TI  - P1.15 CONDITIONAL INACTIVATION OF INTEGRIN AV SUBUNIT IN VASCULAR SMOOTH MUSCLE CELLS REGULATES FIBROSIS IN VESSELS
JO  - Artery Research
SP  - 6
EP  - 6
VL  - 12
IS  - C
SN  - 1876-4401
UR  - https://doi.org/10.1016/j.artres.2015.10.207
DO  - https://doi.org/10.1016/j.artres.2015.10.207
ID  - Belozertseva*2015
ER  -