Artery Research

Volume 20, Issue C, December 2017, Pages 85 - 85

P173 COUPLED NITROSO-SULFIDE SIGNALIZATION TRIGGERS SPECIFIC VASOACTIVE EFFECTS IN INTRARENAL ARTERIES OF PATIENTS WITH ARTERIAL HYPERTENSION

Authors
Sona Cacanyiova1, Andrea Berenyiova1, Frantisek Kristek1, Marian Grman2, Karol Ondrias2, Jan Breza3, Jan Breza3
1Institute of Normal and Pathological Physiology, Slovak Academy of Sciences, Slovak Republic
2Institute of Clinical and Translational Research, Biomedical Research Centre, Slovak Academy of Sciences, Slovak Republic
3Department of Urology, Derer’s University Hospital, Slovak Republic
Available Online 6 December 2017.
DOI
10.1016/j.artres.2017.10.121How to use a DOI?
Abstract

In normotensive conditions, it has been confirmed that S-nitrosothiols, as a source of NO, interact with hydrogen sulfide (H2S) and create new substance/s with specific vasoactive effects. This interaction could represent new regulator pathway also in hypertension. The aim of the study was to investigate the vasoactive effects of H2S, GSNO, and products of H2S/GSNO interaction in lobar arteries isolated from kidney after nephrectomy of patients suffering from arterial hypertension.

Changes in isometric tension after pre-contraction were evaluated. Acetylcholine- induced vasorelaxation was significantly reduced compared to the effect induced by exogenous NO donor, sodium nitroprusside, probably suggesting an endothelium dysfunction. While 1 μmol/l Na2S had a minimal effect on the vascular tone, 20 μmol/l evoked a slight vasorelaxation. GSNO at 0.1 μmol/l induced vasorelaxation which was significantly smaller compared to the effect induced by 1μmol/l. The mixture of GSNO (0.1 μmol/l) and Na2S (1 μmol/l) induced significantly higher vasorelaxation compared to GSNO (0.1 μmol/l) alone only in 5th minute without the differences in the speed.

On the other hand, the mixture prepared from higher concentrations of GSNO (1 μmol/l) and Na2S (10 μmol/l) induced a significantly higher (in 1st, 2nd, 5th, 10th minute) and faster vasorelaxation compared to the effect induced by GSNO (1 μmol/l) alone.

In conditions of arterial hypertension H2S in interaction with GSNO regulated a vasoconstrictor-increased arterial tone towards of more pronounced vasorelaxation compared to GSNO alone. We confirmed for the first time that specific vasoactive effects of coupled nitroso-sulfide signalization were triggered also in human arterial tissue.

Open Access
This is an open access article distributed under the CC BY-NC license.

References

1. Supported: VEGA 2/0074/14, APVV-15-0565, APVV-15-037
Journal
Artery Research
Volume-Issue
20 - C
Pages
85 - 85
Publication Date
2017/12/06
ISSN (Online)
1876-4401
ISSN (Print)
1872-9312
DOI
10.1016/j.artres.2017.10.121How to use a DOI?
Open Access
This is an open access article distributed under the CC BY-NC license.

Cite this article

TY  - JOUR
AU  - Sona Cacanyiova
AU  - Andrea Berenyiova
AU  - Frantisek Kristek
AU  - Marian Grman
AU  - Karol Ondrias
AU  - Jan Breza
AU  - Jan Breza
PY  - 2017
DA  - 2017/12/06
TI  - P173 COUPLED NITROSO-SULFIDE SIGNALIZATION TRIGGERS SPECIFIC VASOACTIVE EFFECTS IN INTRARENAL ARTERIES OF PATIENTS WITH ARTERIAL HYPERTENSION
JO  - Artery Research
SP  - 85
EP  - 85
VL  - 20
IS  - C
SN  - 1876-4401
UR  - https://doi.org/10.1016/j.artres.2017.10.121
DO  - 10.1016/j.artres.2017.10.121
ID  - Cacanyiova2017
ER  -