Artery Research

Volume 20, Issue C, December 2017, Pages 74 - 75

P78 LONGITUDINAL MICRO-ULTRASOUND ASSESSMENT OF THE OB/OB MOUSE MODEL: EVALUATION OF CARDIOVASCULAR, RENAL AND HEPATIC PARAMETERS

Authors
Nicole Di Lascio1, 2, Claudia Kusmic1, Francesco Stea3, Francesca Lenzarini1, Cristina Barsanti1, Arthur Leloup4, Francesco Faita1
1Institute of Clinical Physiology, CNR, Pisa, Italy
2Institute of Life Sciences, Scuola Superiore Sant’Anna, Pisa, Italy
3Department of Clinical and Experimental Medicine, University of Pisa, Pisa, Italy
4Department of Pharmaceutical Sciences, University of Antwerp, Antwerp, Belgium
Available Online 6 December 2017.
DOI
10.1016/j.artres.2017.10.094How to use a DOI?
Abstract

Purpose/Background/Objectives: Obesity is associated with increased risk factors for cardiovascular disease (CVD). Leptin-deficient mice (ob/ob) are widely employed to investigate obesity. This study is aimed at providing a micro-ultrasound (mUS) longitudinal evaluation of the ob/ob mouse in terms of cardiovascular effects, consequences for renal microcirculation and liver fat accumulation.

Methods: Sixteen wild-type (wt) and eleven ob/ob male mice were studied at 8 (T0) and 25 (T1) weeks of age with a mUS system (Vevo2100). Cardiac output (CO), ejection fraction (EF), stroke volume (SV), fractional shortening (FS) and E/A ratio were measured from cardiac images. Mean diameter (Dmabd, Dmcar), relative distension (relDabd, relDcar) and pulse wave velocity (PWVabd, PWVcar) were obtained for both abdominal aorta and common carotid. Renal resistivity and pulsatility index (RI, PI) were assessed. The ratio between grey-levels related to liver and kidney (Steato-Score) was used as index of hepatic steatosis.

Results: At T0, ob/ob mice showed reduced SV, EF, CO and relDabd values and increased LVmass, PWVabd, RI, PI and Steato-score. Similar results for SV, EF, CO, RI, PI and Steato-Score were found at T1; furthermore, obese mice showed reduced Dmabd and Dmcar measurements in comparison with lean controls. The longitudinal analysis showed an increase in LVmass and Dmabd and a reduction of FS, EF, CO, relDabd and relDcar for wt animals and no differences for the ob/ob one.

Conclusions: ob/ob mice presented a premature cardiac dysfunction without a further age-related deterioration and a reduction in the abdominal aorta and carotid artery mean diameter in adult age.

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Journal
Artery Research
Volume-Issue
20 - C
Pages
74 - 75
Publication Date
2017/12/06
ISSN (Online)
1876-4401
ISSN (Print)
1872-9312
DOI
10.1016/j.artres.2017.10.094How to use a DOI?
Open Access
This is an open access article distributed under the CC BY-NC license.

Cite this article

TY  - JOUR
AU  - Nicole Di Lascio
AU  - Claudia Kusmic
AU  - Francesco Stea
AU  - Francesca Lenzarini
AU  - Cristina Barsanti
AU  - Arthur Leloup
AU  - Francesco Faita
PY  - 2017
DA  - 2017/12/06
TI  - P78 LONGITUDINAL MICRO-ULTRASOUND ASSESSMENT OF THE OB/OB MOUSE MODEL: EVALUATION OF CARDIOVASCULAR, RENAL AND HEPATIC PARAMETERS
JO  - Artery Research
SP  - 74
EP  - 75
VL  - 20
IS  - C
SN  - 1876-4401
UR  - https://doi.org/10.1016/j.artres.2017.10.094
DO  - 10.1016/j.artres.2017.10.094
ID  - DiLascio2017
ER  -