Artery Research

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Volume 20, Issue C, December 2017, Pages 61 - 61

5.7 SUBPOPULATIONS OF CIRCULATING T LYMPHOCYTES IN OBESE PATIENTS UNDERGOING BARIATRIC SURGERY

Authors
C. Agabiti Rosei1, C. Rossini1, F. Mittempergher2, A. Titi2, N. Portolani2, C. De Ciuceis1, S. Caletti1, M.A. Coschignano1, V. Trapletti1, E. Porteri1, P. Pileri1, E. Agabiti Rosei1, D. Rizzoni1, 3
1Clinica Medica, Department of Clinical and Experimental Sciences, University of Brescia, Italy
2Clinica Chirurgica, Department of Clinical and Experimental Sciences, University of Brescia, Italy
3Istituto Clinico Città di Brescia, Division of Medicine, Brescia, Italy
Available Online 6 December 2017.
DOI
10.1016/j.artres.2017.10.053How to use a DOI?
Abstract

Objective: It has been previously demonstrated that T lymphocytes may be involved in the development of hypertension and microvascular remodeling, and that circulating T effector lymphocytes may be increased in hypertension. In particular, Th1 and Th17 lymphocytes may contribute to the progression of hypertension and microvascular damage while TREG lymphocytes seem to be protective. However, no data is available about patients with severe obesity, in which pronounced microvascular alterations were observed.

Methods: We have investigated 32 severely obese patients undergoing bariatric surgery, 24 normotensive lean subjects and 11 hypertensive lean subjects undergoing an election surgical intervention. No sign of local or systemic inflammation was present in any subject or patient. A peripheral blood sample was obtained before surgery for assessment of CD4+ T lymphocyte subpopulations. Lymphocyte phenotype was evaluated by flow cytometry after 5 hour in vitro activation in order to assess T-effector and T-regulatory (TREG) lymphocytes. Subsets of TREGS were defined as follows: −TREGS recent thymic emigrants (RTE), directly derived from thymus: CD31+; −TREGS naïve: CCR7+CD45RA+; −TREGS central memory (CM): CCR7+CD45RA−; −TREGS effector memory (EM): CCR7−CD45RA−; −TREGS terminal differentiated effector memory (TDEM): CCR7−CD45RA+.

Results: The results are summarized in the Table (*p < 0.05, **p < 0.01, ***p < 0.001 vs. lean normotensives; #p < 0.05, ##p < 0.01, ###p < 0.001 vs. lean hypertensives). A marked reduction of several TREG subpopulations was observed in obese patients compared with controls, together with an increased in some T-effector cells.

Lean normotensives Lean hypertensives Obese patients
TREGs (%) 4.11 ± 1.60 4.64 ± 1.66 2.69 ± 1.81**##
TREGs (abs number) 45.4 ± 24.3 45.4 ± 23.8 27.3 ± 21.1**#
TREGs naíve (%) 22.1 ± 10.1 18.1 ± 13.1 13.34 ± 12.9**
TREGs naíve (abs number) 10.6 ± 7.75 9.71 ± 8.87 3.87 ± 5.28***##
TREG CM (%) 32.3 ± 13.8 32.8 ± 17.8 22.7 ± 15.2*#
TREGs CM (abs number) 14.7 ± 10.2 14.2 ± 9.08 6.10 ± 8.08***##
CD4+ EM (%) 24.4 ± 9.96 26.8 ± 12.5 34.1 ± 13.3**
CD161+CD28+ (%) 86.2 ± 28.5 94.9 ± 5.63 97.2 ± 5.39*

Conclusion: TREG lymphocytes are clearly reduced in severely obese patients, possibly contributing to the development of marked microvascular alterations previously observed in such a population.

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Journal
Artery Research
Volume-Issue
20 - C
Pages
61 - 61
Publication Date
2017/12/06
ISSN (Online)
1876-4401
ISSN (Print)
1872-9312
DOI
10.1016/j.artres.2017.10.053How to use a DOI?
Open Access
This is an open access article distributed under the CC BY-NC license.

Cite this article

risenwbib
TY  - JOUR
AU  - C. Agabiti Rosei
AU  - C. Rossini
AU  - F. Mittempergher
AU  - A. Titi
AU  - N. Portolani
AU  - C. De Ciuceis
AU  - S. Caletti
AU  - M.A. Coschignano
AU  - V. Trapletti
AU  - E. Porteri
AU  - P. Pileri
AU  - E. Agabiti Rosei
AU  - D. Rizzoni
PY  - 2017
DA  - 2017/12/06
TI  - 5.7 SUBPOPULATIONS OF CIRCULATING T LYMPHOCYTES IN OBESE PATIENTS UNDERGOING BARIATRIC SURGERY
JO  - Artery Research
SP  - 61
EP  - 61
VL  - 20
IS  - C
SN  - 1876-4401
UR  - https://doi.org/10.1016/j.artres.2017.10.053
DO  - 10.1016/j.artres.2017.10.053
ID  - AgabitiRosei2017
ER  -